FLGA2_HALVD
ID FLGA2_HALVD Reviewed; 220 AA.
AC D4GWY2; L9UTC9;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 25-MAY-2022, entry version 57.
DE RecName: Full=Flagellin A2;
DE Flags: Precursor;
GN Name=flgA2; OrderedLocusNames=HVO_1211; ORFNames=C498_12693;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=10632878; DOI=10.1046/j.1365-2958.2000.01677.x;
RA Tarasov V.Y., Pyatibratov M.G., Tang S.L., Dyall-Smith M., Fedorov O.V.;
RT "Role of flagellins from A and B loci in flagella formation of
RT Halobacterium salinarum.";
RL Mol. Microbiol. 35:69-78(2000).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20363933; DOI=10.1128/jb.00133-10;
RA Tripepi M., Imam S., Pohlschroeder M.;
RT "Haloferax volcanii flagella are required for motility but are not involved
RT in PibD-dependent surface adhesion.";
RL J. Bacteriol. 192:3093-3102(2010).
RN [5]
RP GLYCOSYLATION.
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=22730124; DOI=10.1128/jb.00731-12;
RA Tripepi M., You J., Temel S., Onder O., Brisson D., Pohlschroder M.;
RT "N-glycosylation of Haloferax volcanii flagellins requires known Agl
RT proteins and is essential for biosynthesis of stable flagella.";
RL J. Bacteriol. 194:4876-4887(2012).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=23989184; DOI=10.1099/mic.0.069617-0;
RA Tripepi M., Esquivel R.N., Wirth R., Pohlschroeder M.;
RT "Haloferax volcanii cells lacking the flagellin FlgA2 are hypermotile.";
RL Microbiology 159:2249-2258(2013).
CC -!- FUNCTION: Flagellin that plays both structural and regulatory roles in
CC flagella biosynthesis. Does not constitute a major flagellin in terms
CC of abundance contrary to FlgA1: may regulate the flagella-dependent
CC swimming motility depending on the relative abundance of FlgA1. Not
CC involved in PibD-dependent surface adhesion.
CC {ECO:0000269|PubMed:20363933, ECO:0000269|PubMed:23989184}.
CC -!- SUBCELLULAR LOCATION: Archaeal flagellum {ECO:0000250}.
CC -!- PTM: Glycosylated by a pentasaccharide similar to the S-layer
CC glycoprotein, probably comprising a hexose, 2 hexuronic acids, a methyl
CC ester of a hexuronic acid and mannose. {ECO:0000269|PubMed:22730124}.
CC -!- DISRUPTION PHENOTYPE: Straight flagella, mainly at the poles
CC (PubMed:10632878). Cells lacking flgA2 but not flgA1 are hypermotile,
CC display an increased number of flagella per cell, as well as an
CC increased flagellum length (PubMed:23989184). Cells lacking both flgA1
CC and flgA2 show defects in motility, whitout affecting surface adhesion
CC ability (PubMed:20363933). {ECO:0000269|PubMed:10632878,
CC ECO:0000269|PubMed:20363933, ECO:0000269|PubMed:23989184}.
CC -!- SIMILARITY: Belongs to the archaeal flagellin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ELY28104.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP001956; ADE03249.1; -; Genomic_DNA.
DR EMBL; AOHU01000091; ELY28104.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_013035369.1; NZ_AOHU01000091.1.
DR AlphaFoldDB; D4GWY2; -.
DR SMR; D4GWY2; -.
DR STRING; 309800.C498_12693; -.
DR EnsemblBacteria; ADE03249; ADE03249; HVO_1211.
DR EnsemblBacteria; ELY28104; ELY28104; C498_12693.
DR GeneID; 8924200; -.
DR KEGG; hvo:HVO_1211; -.
DR PATRIC; fig|309800.29.peg.2431; -.
DR eggNOG; arCOG01829; Archaea.
DR HOGENOM; CLU_051124_1_0_2; -.
DR OMA; MIELFND; -.
DR OrthoDB; 94462at2157; -.
DR Proteomes; UP000008243; Chromosome.
DR Proteomes; UP000011532; Unassembled WGS sequence.
DR GO; GO:0097589; C:archaeal-type flagellum; IEA:UniProtKB-SubCell.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0097588; P:archaeal or bacterial-type flagellum-dependent cell motility; IEA:InterPro.
DR InterPro; IPR013373; Flagellin/pilin_N.
DR InterPro; IPR002774; Flagellin_arc.
DR PANTHER; PTHR35903; PTHR35903; 1.
DR Pfam; PF01917; Arch_flagellin; 1.
DR TIGRFAMs; TIGR02537; arch_flag_Nterm; 1.
PE 1: Evidence at protein level;
KW Archaeal flagellum; Glycoprotein; Reference proteome.
FT CHAIN 1..220
FT /note="Flagellin A2"
FT /id="PRO_0000429054"
FT PROPEP 1..11
FT /evidence="ECO:0000255"
FT /id="PRO_0000429055"
FT CARBOHYD 78
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 220 AA; 22810 MW; 8F87AC8E05F80C90 CRC64;
MFNNITDDDR GQVGIGTLIV FIAMVLVAAI AAGVLVNTAG FLQATAEDAG EQSVNKVTNR
VEVLNTHGTV GGEEDIDNIT LTVRLAAGSD AVDMNETSIK YLSGDSVVTL TNQTVTSGAN
SATNDSAEGV ADSDEFGLSE VTDDDGSFGV LNSMNDRYEV TIDTAAIETS DGDNTNLIGG
LSTGEQVTLE ITSRTGGTTQ VILTMPQQLA GKTQNEPVEL
