FG3H_SOYBN
ID FG3H_SOYBN Reviewed; 459 AA.
AC I1KEV6;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=UDP-glycosyltransferase 79B30 {ECO:0000303|PubMed:26002063};
DE EC=2.4.1.239 {ECO:0000305};
DE AltName: Full=Flavonol 3-O-glucoside/galactoside (1->2) glucosyltransferase {ECO:0000303|PubMed:26002063};
DE Short=GmF3G2''Gt-b {ECO:0000303|PubMed:26002063};
GN Name=FG3 {ECO:0000303|PubMed:26002063};
GN Synonyms=UGT79B30 {ECO:0000303|PubMed:26002063};
GN OrderedLocusNames=Glyma06g43880;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000312|Proteomes:UP000008827};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, 3D-STRUCTURE
RP MODELING, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Harosoy;
RX PubMed=26002063; DOI=10.1186/s12870-015-0504-7;
RA Di S., Yan F., Rodas F.R., Rodriguez T.O., Murai Y., Iwashina T.,
RA Sugawara S., Mori T., Nakabayashi R., Yonekura-Sakakibara K., Saito K.,
RA Takahashi R.;
RT "Linkage mapping, molecular cloning and functional analysis of soybean gene
RT Fg3 encoding flavonol 3-O-glucoside/galactoside (1->2)
RT glucosyltransferase.";
RL BMC Plant Biol. 15:126-126(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82;
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
CC -!- FUNCTION: Flavonol 3-O-glucoside/galactoside (1->2) glucosyltransferase
CC converting kaempferol 3-O-glucoside to kaempferol 3-O-sophoroside. Has
CC a broad in vitro activity for kaempferol/ quercetin 3-O-
CC glucoside/galactoside derivatives, but cannot glucosylate kaempferol 3-
CC O-rhamnosyl-(1->4)-[rhamnosyl-(1->6)- glucoside] and 3-O-rhamnosyl-
CC (1->4)-[glucosyl-(1->6)-glucoside]. Has a higher preference for UDP-
CC glucose than UDP-galactose, and no activity with UDP-arabinose and UDP-
CC glucuronic acid. Represents probably a recessive allele of the gene.
CC {ECO:0000269|PubMed:26002063}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a flavonol 3-O-beta-D-glucoside + UDP-alpha-D-glucose = a
CC flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside + H(+) + UDP;
CC Xref=Rhea:RHEA:18893, ChEBI:CHEBI:15378, ChEBI:CHEBI:16816,
CC ChEBI:CHEBI:52142, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC EC=2.4.1.239; Evidence={ECO:0000305};
CC -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:26002063}.
CC -!- MISCELLANEOUS: Flavonol glycosides (FGs) having glucose at the 2''-
CC position of glucose or galactose that is bound to the 3-position of
CC kaempferol are present in cv. Nezumisaya and absent in cv. Harosoy,
CC whereas FGs having glucose at the 6''-position of glucose or galactose
CC that is bound to the 3-position of kaempferol are present in cv.
CC Harosoy and absent in cv. Nezumisaya. {ECO:0000269|PubMed:26002063}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; LC017845; BAR88078.1; -; mRNA.
DR EMBL; CM000839; KRH55845.1; -; Genomic_DNA.
DR AlphaFoldDB; I1KEV6; -.
DR SMR; I1KEV6; -.
DR STRING; 3847.GLYMA06G43880.1; -.
DR PRIDE; I1KEV6; -.
DR EnsemblPlants; KRH55845; KRH55845; GLYMA_06G285700.
DR Gramene; KRH55845; KRH55845; GLYMA_06G285700.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_2_3_1; -.
DR InParanoid; I1KEV6; -.
DR OMA; YTKDNVC; -.
DR OrthoDB; 508327at2759; -.
DR BRENDA; 2.4.1.239; 2483.
DR Proteomes; UP000008827; Chromosome 6.
DR GO; GO:0033838; F:flavonol-3-O-glucoside glucosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..459
FT /note="UDP-glycosyltransferase 79B30"
FT /id="PRO_0000436354"
SQ SEQUENCE 459 AA; 51109 MW; 7FA6D03D5F39A900 CRC64;
MKSRPLHIAM YPWLAMGHQT AFLHLCNKLA IRGHKISFIT PPKAQAKLEP FNLHPNSITF
VTINVPHVEG LPPDAQTTAD VTYPLQPQIM TAMDLTKDDI ETLLTGLKPD LVFYDFTHWM
PALAKRLGIK AVHYCTASSV MVGYTLTPSR FHQGTDLMES DLMEPPEGYP DSSIKLQTHE
ARTFAAKRKD TFGSNVLFYD RQFIALNEAD LLAYRTCREI EGPYMDYIGK QFNKPVVATG
PVILDPPTLD LEEKFSTWLG GFEPGSVVYC CFGSECTLRP NQFLELVLGL ELTGMPFLAA
VKAPLGFETV ESAMPEGFQE RVKGRGFVYG GWVQQQLILA HPSVGCFITH CGSGSLSEAL
VNKCQLVLLP NVGDQILNAR MMGTNLEVGV EVEKGDEDGM YTKESVCKAV SIVMDCENET
SKRVRANHAR IRELLLNKDL ESSYVDSFCM RLQEIVEGI
