ID   AK1CO_HORSE             Reviewed;         323 AA.
AC   Q6W8P9;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Aldo-keto reductase family 1 member C23-like protein;
DE            EC=1.1.1.-;
DE   AltName: Full=Putative prostaglandin F synthase;
GN   Name=PGFS;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Ovary;
RX   PubMed=14561653; DOI=10.1095/biolreprod.103.020800;
RA   Boerboom D., Brown K.A., Vaillancourt D., Poitras P., Goff A.K.,
RA   Watanabe K., Dore M., Sirois J.;
RT   "Expression of key prostaglandin synthases in equine endometrium during
RT   late diestrus and early pregnancy.";
RL   Biol. Reprod. 70:391-399(2004).
CC   -!- FUNCTION: NADP-dependent oxidoreductase involved in steroid metabolism.
CC       May act on various hydroxysteroids.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Detected in endometrium surface epithelium (at
CC       protein level). Detected in endometrium. {ECO:0000269|PubMed:14561653}.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; AY304536; AAP69945.1; -; mRNA.
DR   RefSeq; NP_001075364.1; NM_001081895.2.
DR   AlphaFoldDB; Q6W8P9; -.
DR   SMR; Q6W8P9; -.
DR   PaxDb; Q6W8P9; -.
DR   PRIDE; Q6W8P9; -.
DR   GeneID; 100034026; -.
DR   KEGG; ecb:100034026; -.
DR   CTD; 100034026; -.
DR   InParanoid; Q6W8P9; -.
DR   OrthoDB; 1016440at2759; -.
DR   Proteomes; UP000002281; Unplaced.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0047023; F:androsterone dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0032052; F:bile acid binding; IBA:GO_Central.
DR   GO; GO:0047086; F:ketosteroid monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0016229; F:steroid dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0044597; P:daunorubicin metabolic process; IBA:GO_Central.
DR   GO; GO:0044598; P:doxorubicin metabolic process; IBA:GO_Central.
DR   GO; GO:0042448; P:progesterone metabolic process; IBA:GO_Central.
DR   GO; GO:0006693; P:prostaglandin metabolic process; IBA:GO_Central.
DR   GO; GO:0008202; P:steroid metabolic process; IBA:GO_Central.
DR   CDD; cd19108; AKR_AKR1C1-35; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044482; AKR1C.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Lipid metabolism; NADP; Oxidoreductase; Reference proteome;
KW   Steroid metabolism.
FT   CHAIN           1..323
FT                   /note="Aldo-keto reductase family 1 member C23-like
FT                   protein"
FT                   /id="PRO_0000326225"
FT   ACT_SITE        55
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         20..24
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         31
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         50
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         166..167
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         190
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         216..222
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         270..280
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            84
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   323 AA;  37040 MW;  9FFCF6C13D35DB3B CRC64;
     MDPKGWCEKL NDGHVIPVLG FGTYAPEEVP KSRTVEVTKL AIDAGFRHID SAYSYNNEKE
     VGQAIRSKIE DGTVKREDIF YTSKLWLTFL RPELVRPALE KSLTNLQLDY VDLYIIHFPI
     ALKPGEELFP EDEHGKLIFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP
     GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAYGALG TQRLKRWLAP NSPVLLEDPV
     LCAMAKKYKR TPALIALRYL LQRGVVVLAK SYNEKRIKEN MQVFEFQLTS EDMKDLDGLN
     RNHRFLPLQI AVDHPEYPFA DEY