ID   AK1A1_XENLA             Reviewed;         327 AA.
AC   Q6GMC7;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Aldo-keto reductase family 1 member A1;
DE            EC=1.1.1.2 {ECO:0000250|UniProtKB:P14550};
DE   AltName: Full=Alcohol dehydrogenase [NADP(+)];
DE   AltName: Full=Aldehyde reductase;
GN   Name=akr1a1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of a wide variety of
CC       carbonyl-containing compounds to their corresponding alcohols. Displays
CC       enzymatic activity towards endogenous metabolites such as aromatic and
CC       aliphatic aldehydes, ketones, monosaccharides and bile acids. Acts as
CC       an aldehyde-detoxification enzyme (By similarity). Displays no
CC       reductase activity towards retinoids (By similarity).
CC       {ECO:0000250|UniProtKB:P14550, ECO:0000250|UniProtKB:P50578,
CC       ECO:0000250|UniProtKB:P51635}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a primary alcohol + NADP(+) = an aldehyde + H(+) + NADPH;
CC         Xref=Rhea:RHEA:15937, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.2;
CC         Evidence={ECO:0000250|UniProtKB:P14550};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9JII6}. Apical cell membrane
CC       {ECO:0000250|UniProtKB:Q9JII6}.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; BC074141; AAH74141.1; -; mRNA.
DR   RefSeq; NP_001086059.1; NM_001092590.1.
DR   AlphaFoldDB; Q6GMC7; -.
DR   SMR; Q6GMC7; -.
DR   DNASU; 444488; -.
DR   GeneID; 444488; -.
DR   KEGG; xla:444488; -.
DR   CTD; 444488; -.
DR   Xenbase; XB-GENE-1014358; akr1a1.L.
DR   OrthoDB; 1016440at2759; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 444488; Expressed in kidney and 20 other tissues.
DR   GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0008106; F:alcohol dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046185; P:aldehyde catabolic process; IEA:InterPro.
DR   GO; GO:0110095; P:cellular detoxification of aldehyde; ISS:UniProtKB.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd19106; AKR_AKR1A1-4; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044481; AKR1A.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasm; Lipid metabolism; Membrane; NADP; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..327
FT                   /note="Aldo-keto reductase family 1 member A1"
FT                   /id="PRO_0000384154"
FT   ACT_SITE        52
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P14550"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P14550"
FT   BINDING         213..275
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            82
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250|UniProtKB:P14550"
SQ   SEQUENCE   327 AA;  37100 MW;  1B7E3388A33B90E2 CRC64;
     MATSVEYETL YTGQKMPLIG LGTWKSAPGQ VKDAVKYALG VGYRHIDCAF VYGNETEVGE
     AIKESVGPDK GLKREEVFVT SKLWNNKHHP DDVECALRKT LQELQLDYLD LYLMHWPYAF
     KRGDQVFPQN PDGSVQYDLT DYKDTWKAME KLVEKGLAKA IGLSNFNKRQ MDDILSIATV
     KPAVLQVECH PYLAQNELIA CCRSHGLVLT GYSPLGSPDR SWRKPEDPVL LEEPGIIAMA
     KKYNISEAQI LLRWQVQRRV VTIPKSVTPA RILQNLQVFD FSLSEEEMQL IGALNKNWRY
     IIPLITVNGK SVPRDAGHPL YPFNDPY