AJUBA_DROME
ID AJUBA_DROME Reviewed; 728 AA.
AC Q9VY77; Q8SZR9;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=LIM domain-containing protein jub;
GN Name=jub; ORFNames=CG11063;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Rubin G.M., Celniker S.;
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH SAV AND WTS.
RX PubMed=20303269; DOI=10.1016/j.cub.2010.02.035;
RA Das Thakur M., Feng Y., Jagannathan R., Seppa M.J., Skeath J.B.,
RA Longmore G.D.;
RT "Ajuba LIM proteins are negative regulators of the Hippo signaling
RT pathway.";
RL Curr. Biol. 20:657-662(2010).
CC -!- FUNCTION: Regulates organ size by inhibiting apoptosis and promoting
CC cell proliferation by influencing the expression of G1/S-specific
CC cyclin-E (CycE) and apoptosis 1 inhibitor (th). Negatively regulates
CC the Hippo signaling pathway. {ECO:0000269|PubMed:20303269}.
CC -!- SUBUNIT: Interacts with sav and wts. {ECO:0000269|PubMed:20303269}.
CC -!- SUBCELLULAR LOCATION: Cell junction, adherens junction
CC {ECO:0000269|PubMed:20303269}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9VY77-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9VY77-2; Sequence=VSP_043063;
CC -!- SIMILARITY: Belongs to the zyxin/ajuba family. {ECO:0000305}.
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DR EMBL; AE014298; AAF48328.3; -; Genomic_DNA.
DR EMBL; AY070556; AAL48027.1; -; mRNA.
DR EMBL; BT014658; AAT27282.1; -; mRNA.
DR RefSeq; NP_572930.3; NM_132702.5. [Q9VY77-1]
DR AlphaFoldDB; Q9VY77; -.
DR BioGRID; 58722; 15.
DR IntAct; Q9VY77; 1.
DR STRING; 7227.FBpp0073665; -.
DR PaxDb; Q9VY77; -.
DR PRIDE; Q9VY77; -.
DR EnsemblMetazoa; FBtr0073834; FBpp0073665; FBgn0030530. [Q9VY77-1]
DR GeneID; 32351; -.
DR KEGG; dme:Dmel_CG11063; -.
DR UCSC; CG11063-RB; d. melanogaster. [Q9VY77-1]
DR CTD; 32351; -.
DR FlyBase; FBgn0030530; jub.
DR VEuPathDB; VectorBase:FBgn0030530; -.
DR eggNOG; KOG1701; Eukaryota.
DR InParanoid; Q9VY77; -.
DR OMA; AHIYAPT; -.
DR PhylomeDB; Q9VY77; -.
DR Reactome; R-DME-1234176; Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
DR SignaLink; Q9VY77; -.
DR BioGRID-ORCS; 32351; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 32351; -.
DR PRO; PR:Q9VY77; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030530; Expressed in embryonic/larval hemocyte (Drosophila) and 21 other tissues.
DR ExpressionAtlas; Q9VY77; baseline and differential.
DR Genevisible; Q9VY77; DM.
DR GO; GO:0005912; C:adherens junction; IDA:FlyBase.
DR GO; GO:0045179; C:apical cortex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0140311; F:protein sequestering activity; IPI:FlyBase.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0007010; P:cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0035331; P:negative regulation of hippo signaling; IMP:FlyBase.
DR GO; GO:0030707; P:ovarian follicle cell development; IMP:FlyBase.
DR GO; GO:0045572; P:positive regulation of imaginal disc growth; IMP:FlyBase.
DR GO; GO:2000637; P:positive regulation of miRNA-mediated gene silencing; IBA:GO_Central.
DR GO; GO:0071539; P:protein localization to centrosome; IMP:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0001666; P:response to hypoxia; IBA:GO_Central.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00412; LIM; 3.
DR SMART; SM00132; LIM; 3.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell junction; LIM domain; Metal-binding;
KW Reference proteome; Repeat; Zinc.
FT CHAIN 1..728
FT /note="LIM domain-containing protein jub"
FT /id="PRO_0000416967"
FT DOMAIN 514..575
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 579..639
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 640..708
FT /note="LIM zinc-binding 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 28..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 287..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 407..427
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..532
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12537569, ECO:0000303|Ref.4"
FT /id="VSP_043063"
SQ SEQUENCE 728 AA; 77961 MW; 86F9CAFF840E4541 CRC64;
MTTQRTQTQA RNPGNSDSDY ETLMQRLHIG GGGGAGVGGG GGSGGPGDGG VAMRSYQRSP
GAIENYLQES QQQQQQHQMQ QMQQLHHNQD YKLYERGNII AASKYATPRP VEQIQHTHNG
SAQIYAPTAQ ILGQRIAPQK HSPVYENLEF YGQFDSNHKR AQPQSPGSRQ SAILNGYLLM
QPIRAGRFAH TPVPENQGSA SLGTTRAAGG PPPPSGRSAA LGPTGDLEEH AAPIYENIIP
HSGQRAQPQA SPATATMYQE MQPTSNSSGS SSTAGLDLNA LLASPMHQRS ISSNTASSSS
LGSPTQRYRN LSLPSHLSPV PTAQSVAKLQ QHTPVKSPGG AINVSVNPNY IEDINSSDYV
CMTANLHRNT AAGLATGRAA ATGAPAQRTV QEPPIITSSL AMGMATAAPP TTSNAPTSAA
AAPAAPTSTA SLRATPIAMT APLAVATSPT PSQGSTAVNA ALKPRRGLTK NLLPYSVTPP
RPAGPTEAQR KIEELTRQLE EEIEQSEEHG EYFGICHTCG EKVKGAGQAC QAMGNLYHTN
CFICCSCGRA LRGKAFYNVH GRVYCEEDYM YSGFQQTAEK CAICGHLIME MILQAMGKSY
HPGCFRCCVC NECLDGVPFT VDVDHKIYCV NDYHRMFAPK CASCGKGITP VEGTDETVRV
VSMDKDFHVD CYICEECGMQ LTDEPDKRCY PLDGRLLCRG CHLQRLALQS SPHARHQEPV
CASYQYMG
