FAS3_DROME
ID FAS3_DROME Reviewed; 508 AA.
AC P15278; Q8SXX9; Q9VJ89;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 192.
DE RecName: Full=Fasciclin-3;
DE AltName: Full=Fasciclin III;
DE Short=FAS III;
DE Flags: Precursor;
GN Name=Fas3; ORFNames=CG5803;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), AND PYROGLUTAMATE FORMATION AT
RP GLN-21.
RX PubMed=2509076; DOI=10.1016/0092-8674(89)90293-6;
RA Snow P.M., Bieber A.J., Goodman C.S.;
RT "Fasciclin III: a novel homophilic adhesion molecule in Drosophila.";
RL Cell 59:313-323(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Mediates cell adhesion in a Ca(2+)-independent manner. It
CC plays a role in axon outgrowth, guidance and fasciculation of the
CC developing nervous system.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=P15278-1; Sequence=Displayed;
CC Name=B;
CC IsoId=P15278-2; Sequence=VSP_007949, VSP_007950;
CC -!- TISSUE SPECIFICITY: Expressed on different subsets of axon bundles
CC (fascicles) in insect embryos.
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DR EMBL; M27813; AAA28532.1; -; mRNA.
DR EMBL; AE014134; AAF53665.2; -; Genomic_DNA.
DR EMBL; AE014134; AAN11002.1; -; Genomic_DNA.
DR EMBL; AY075516; AAL68324.1; -; mRNA.
DR PIR; A33378; A33378.
DR RefSeq; NP_001286039.1; NM_001299110.1. [P15278-2]
DR RefSeq; NP_724106.1; NM_165249.4. [P15278-2]
DR RefSeq; NP_724107.1; NM_165250.4. [P15278-1]
DR AlphaFoldDB; P15278; -.
DR BioGRID; 61092; 13.
DR DIP; DIP-21336N; -.
DR STRING; 7227.FBpp0111717; -.
DR GlyGen; P15278; 3 sites.
DR SwissPalm; P15278; -.
DR PaxDb; P15278; -.
DR DNASU; 35097; -.
DR EnsemblMetazoa; FBtr0081051; FBpp0080604; FBgn0000636. [P15278-1]
DR EnsemblMetazoa; FBtr0081052; FBpp0080605; FBgn0000636. [P15278-2]
DR EnsemblMetazoa; FBtr0340580; FBpp0309467; FBgn0000636. [P15278-2]
DR GeneID; 35097; -.
DR KEGG; dme:Dmel_CG5803; -.
DR CTD; 35097; -.
DR FlyBase; FBgn0000636; Fas3.
DR VEuPathDB; VectorBase:FBgn0000636; -.
DR eggNOG; ENOG502QWSY; Eukaryota.
DR GeneTree; ENSGT00940000169499; -.
DR InParanoid; P15278; -.
DR PhylomeDB; P15278; -.
DR BioGRID-ORCS; 35097; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Fas3; fly.
DR GenomeRNAi; 35097; -.
DR PRO; PR:P15278; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0000636; Expressed in wing disc and 48 other tissues.
DR ExpressionAtlas; P15278; baseline and differential.
DR Genevisible; P15278; DM.
DR GO; GO:0005911; C:cell-cell junction; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0031594; C:neuromuscular junction; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0055037; C:recycling endosome; IDA:FlyBase.
DR GO; GO:0005920; C:smooth septate junction; IDA:FlyBase.
DR GO; GO:0050839; F:cell adhesion molecule binding; IBA:GO_Central.
DR GO; GO:0007411; P:axon guidance; IEP:FlyBase.
DR GO; GO:0007413; P:axonal fasciculation; TAS:FlyBase.
DR GO; GO:0098609; P:cell-cell adhesion; IBA:GO_Central.
DR GO; GO:0007156; P:homophilic cell adhesion via plasma membrane adhesion molecules; TAS:FlyBase.
DR GO; GO:0008039; P:synaptic target recognition; IDA:FlyBase.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR013162; CD80_C2-set.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF08205; C2-set_2; 1.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell adhesion; Developmental protein;
KW Differentiation; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Neurogenesis; Phosphoprotein; Pyrrolidone carboxylic acid;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT CHAIN 21..508
FT /note="Fasciclin-3"
FT /id="PRO_0000014760"
FT TOPO_DOM 21..346
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..508
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 44..106
FT /note="Ig-like V-type"
FT DOMAIN 126..223
FT /note="Ig-like C2-type 1"
FT DOMAIN 236..310
FT /note="Ig-like C2-type 2"
FT REGION 381..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 383..412
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 21
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000305|PubMed:2509076"
FT MOD_RES 382
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT MOD_RES 459
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT CARBOHYD 160
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 257
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 300
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 150..211
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 391..401
FT /note="SDTESADIKAT -> KTNNSSMLNLY (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_007949"
FT VAR_SEQ 402..508
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_007950"
SQ SEQUENCE 508 AA; 55884 MW; 6E39EA0580697D4F CRC64;
MSRIVFICLA AILTDALTWA QVNVEPNTAL LNEGDRTELL CRYGRSINYC RIEIPGEQKV
LNLSPEWSKT PGFTYFGAGL TAGQCGVSIE RVKASNNGQV KCSLGVEGEE LSGTIDLVVA
LRPQQPIIEL LSRPNREGYF NEGTEFRARC SVRDGRPPAN ISWYIDNMPA NKRTTPLEVM
SSTNDNVELS TSVQEIQWHL SPEDSNRKLV CRSHHQTDRE SVPPQEAAYI INVRYAPVHQ
PDAAVYGLYL EHTAIVNITI RASPQPKIEW TIDGAIVGQG RTDGRYSAYE PQYLGNDEYN
VTLAIAGLTL EDTTKIYNLR ASNELGLTDY QVRISSSSKP PSSSLDVAAI VGIVVAVAVL
VLVVLLIVFA RATGRWCFGG KSIKTPTNET SDTESADIKA TSTATATTTM GGVGVSAEEE
ETVNEQESPQ EQQQQQQKKA KRLPAFAAAI LRRFNEKDSR KYKDNQESLN IVEGSVQEIP
ATNNAIDGND NEPKAIVWQS TSPVWTFK
