ERF86_ARATH
ID ERF86_ARATH Reviewed; 348 AA.
AC Q6J9Q2;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ethylene-responsive transcription factor ERF086;
GN Name=ERF086 {ECO:0000303|PubMed:16407444};
GN Synonyms=PUCHI {ECO:0000303|PubMed:17630277}; OrderedLocusNames=At5g18560;
GN ORFNames=T28N17.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 20-348.
RA Pan Y., Gong W., Liu D., Fu Q., Mei W.-Q., Song W.-Q., Ma L.-G., Luo J.-C.,
RA Deng X.-W., Zhu Y.-X.;
RT "Molecular cloning, expression, phylogenetic and functional
RT characterization of the Arabidopsis AP2/EREBP transcription factor
RT family.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION BY AUXIN,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=17630277; DOI=10.1105/tpc.107.050674;
RA Hirota A., Kato T., Fukaki H., Aida M., Tasaka M.;
RT "The auxin-regulated AP2/EREBP gene PUCHI is required for morphogenesis in
RT the early lateral root primordium of Arabidopsis.";
RL Plant Cell 19:2156-2168(2007).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=19482972; DOI=10.1105/tpc.109.067025;
RA Karim M.R., Hirota A., Kwiatkowska D., Tasaka M., Aida M.;
RT "A role for Arabidopsis PUCHI in floral meristem identity and bract
RT suppression.";
RL Plant Cell 21:1360-1372(2009).
RN [7]
RP FUNCTION, AND INDUCTION BY AUXIN.
RX PubMed=23749813; DOI=10.1093/pcp/pct081;
RA Kang N.Y., Lee H.W., Kim J.;
RT "The AP2/EREBP gene PUCHI Co-Acts with LBD16/ASL18 and LBD18/ASL20
RT downstream of ARF7 and ARF19 to regulate lateral root development in
RT Arabidopsis.";
RL Plant Cell Physiol. 54:1326-1334(2013).
CC -!- FUNCTION: Probably acts as a transcriptional activator. May be involved
CC in the regulation of gene expression by stress factors and by
CC components of stress signal transduction pathways (By similarity).
CC Involved in the control of cell division patterns during the early
CC lateral root primordium development. Acts downstream of auxin signaling
CC (PubMed:17630277). Regulated by ARF7 and ARF19 in response to auxin.
CC Co-acts with LBD16 and LBD18 to control lateral root development
CC (PubMed:23749813). Involved in the determination of floral meristem
CC identity and suppression of bract growth. Required for proper
CC conversion of secondary inflorescences to flowers. Acts together with
CC NPR5/BOP2 and NPR6/BOP1 to promote expression of LFY and AP1, two
CC central regulators of floral meristem identity (PubMed:19482972).
CC {ECO:0000250|UniProtKB:Q9LND1, ECO:0000269|PubMed:17630277,
CC ECO:0000269|PubMed:19482972, ECO:0000269|PubMed:23749813}.
CC -!- INTERACTION:
CC Q6J9Q2; Q9MAI5: ERF8; NbExp=3; IntAct=EBI-15202888, EBI-2000137;
CC Q6J9Q2; Q9C5X0: IAA34; NbExp=3; IntAct=EBI-15202888, EBI-3946459;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17630277}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the early stages of lateral root
CC primordia formation (PubMed:17630277). Expressed in early floral
CC meristem (stage 1 to 2) (PubMed:19482972).
CC {ECO:0000269|PubMed:17630277, ECO:0000269|PubMed:19482972}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:17630277,
CC ECO:0000269|PubMed:23749813}.
CC -!- DISRUPTION PHENOTYPE: Disturbed cell division patterns in lateral root
CC primordia leading to the expansion of the proximal region of lateral
CC roots. Short lateral roots in young seedlings (PubMed:17630277). In the
CC shoot, formation of small pin-shaped protrusions at the base of
CC pedicels (PubMed:17630277, PubMed:19482972).
CC {ECO:0000269|PubMed:17630277, ECO:0000269|PubMed:19482972}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AC069328; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92579.1; -; Genomic_DNA.
DR EMBL; AY560873; AAT44940.1; -; mRNA.
DR RefSeq; NP_197357.2; NM_121861.3.
DR AlphaFoldDB; Q6J9Q2; -.
DR SMR; Q6J9Q2; -.
DR BioGRID; 17250; 15.
DR IntAct; Q6J9Q2; 14.
DR STRING; 3702.AT5G18560.1; -.
DR PaxDb; Q6J9Q2; -.
DR PRIDE; Q6J9Q2; -.
DR ProteomicsDB; 220630; -.
DR EnsemblPlants; AT5G18560.1; AT5G18560.1; AT5G18560.
DR GeneID; 831974; -.
DR Gramene; AT5G18560.1; AT5G18560.1; AT5G18560.
DR KEGG; ath:AT5G18560; -.
DR Araport; AT5G18560; -.
DR TAIR; locus:2182993; AT5G18560.
DR eggNOG; ENOG502QQPC; Eukaryota.
DR HOGENOM; CLU_073466_1_0_1; -.
DR InParanoid; Q6J9Q2; -.
DR OMA; IMEPQNF; -.
DR OrthoDB; 1363702at2759; -.
DR PRO; PR:Q6J9Q2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6J9Q2; baseline and differential.
DR Genevisible; Q6J9Q2; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010432; P:bract development; IMP:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010582; P:floral meristem determinacy; IMP:TAIR.
DR GO; GO:0010451; P:floral meristem growth; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0010102; P:lateral root morphogenesis; IMP:TAIR.
DR GO; GO:1901332; P:negative regulation of lateral root development; IGI:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Ethylene signaling pathway; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..348
FT /note="Ethylene-responsive transcription factor ERF086"
FT /id="PRO_0000290409"
FT DNA_BIND 54..111
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 29..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 348 AA; 38455 MW; D9017FC0932CE4BC CRC64;
MSTSKTLDHN KPFETSQTQM GFALIHQNTS ANTTTTTTTG ERRGRRSKQA EPGRFLGVRR
RPWGRYAAEI RDPTTKERHW LGTFDTAHEA ALAYDRAALS MRGTQARTNF VYTPTDVHTI
LTNPNLHSLI VSPYNNNQSF LPNSSPQFVI DHHPHYQNYH QPQQPKHTLP QTVLPAASFK
TPVRHQSVDI QAFGNSPQNS SSNGSLSSSL DEENNFFFSL TSEEHNKSNN NSGYLDCIVP
NHCLKPPPEA TTTQNQAGAS FTTPVASKAS EPYGGFSNSY FEDGEMMMMN HHEFGSCDLS
AMITNYGAAA ASMSMEDYGM MEPQDLSSSS IAAFGDVVAD TTGFYSVF
