ERF82_ARATH
ID ERF82_ARATH Reviewed; 225 AA.
AC O80339; Q0WS49; Q1LYT1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Ethylene-responsive transcription factor 3;
DE Short=AtERF3;
DE AltName: Full=Ethylene-responsive element-binding factor 3;
DE Short=EREBP-3;
GN Name=ERF3; Synonyms=ERF-3, ERF082; OrderedLocusNames=At1g50640;
GN ORFNames=F11F12.4, F17J6.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND INDUCTION.
RX PubMed=10715325; DOI=10.2307/3870944;
RA Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.;
RT "Arabidopsis ethylene responsive element binding factors act as
RT transcriptional activators or repressors of GCC box mediated gene
RT expression.";
RL Plant Cell 12:393-404(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=9756931; DOI=10.1074/jbc.273.41.26857;
RA Hao D., Ohme-Takagi M., Sarai A.;
RT "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-
RT responsive element-binding factor (ERF domain) in plant.";
RL J. Biol. Chem. 273:26857-26861(1998).
RN [7]
RP FUNCTION, AND DOMAIN.
RX PubMed=11487705; DOI=10.2307/3871331;
RA Ohta M., Matsui K., Hiratsu K., Shinshi H., Ohme-Takagi M.;
RT "Repression domains of class II ERF transcriptional repressors share an
RT essential motif for active repression.";
RL Plant Cell 13:1959-1968(2001).
RN [8]
RP INDUCTION.
RX PubMed=12805630; DOI=10.1104/pp.102.017814;
RA Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.;
RT "A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene
RT of Arabidopsis.";
RL Plant Physiol. 132:1020-1032(2003).
RN [9]
RP INTERACTION WITH SAP18.
RX PubMed=16429262; DOI=10.1007/s11103-005-3880-9;
RA Song C.-P., Galbraith D.W.;
RT "AtSAP18, an orthologue of human SAP18, is involved in the regulation of
RT salt stress and mediates transcriptional repression in Arabidopsis.";
RL Plant Mol. Biol. 60:241-257(2006).
RN [10]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Acts as a transcriptional repressor. Binds to the GCC-box
CC pathogenesis-related promoter element. Involved in the regulation of
CC gene expression by stress factors and by components of stress signal
CC transduction pathways and could also regulate other AtERFs.
CC {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:11487705,
CC ECO:0000269|PubMed:9756931}.
CC -!- SUBUNIT: Interacts with SAP18. {ECO:0000269|PubMed:16429262}.
CC -!- INTERACTION:
CC O80339; O64644: At2g45640; NbExp=4; IntAct=EBI-965993, EBI-965964;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O80339-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O80339-2; Sequence=VSP_023535, VSP_023536;
CC -!- INDUCTION: Induced by jasmonate (JA), ethylene and Alternaria
CC brassicicola (locally and systemically). Moderate induction by wounding
CC or drought stress does not require EIN2, whereas induction by NaCl
CC does. Transcripts accumulate slightly in cycloheximide-treated plants,
CC a protein synthesis inhibitor. Seems to not be influenced by ethylene,
CC exogenous abscisic acid (ABA), cold and heat stress.
CC {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:12805630}.
CC -!- DOMAIN: Contains a slightly degenerated ERF-associated amphiphilic
CC repression (EAR) motif, which may be involved in the activity of
CC transcriptional repression. {ECO:0000269|PubMed:11487705}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AB008105; BAA32420.1; -; mRNA.
DR EMBL; AC012561; AAF87871.1; -; Genomic_DNA.
DR EMBL; AC079279; AAG51201.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32574.1; -; Genomic_DNA.
DR EMBL; BT025295; ABF19048.1; -; mRNA.
DR EMBL; AK228091; BAF00050.1; -; mRNA.
DR PIR; T52011; T52011.
DR RefSeq; NP_175479.1; NM_103946.3. [O80339-1]
DR AlphaFoldDB; O80339; -.
DR SMR; O80339; -.
DR BioGRID; 26711; 9.
DR IntAct; O80339; 6.
DR STRING; 3702.AT1G50640.1; -.
DR PaxDb; O80339; -.
DR PRIDE; O80339; -.
DR EnsemblPlants; AT1G50640.1; AT1G50640.1; AT1G50640. [O80339-1]
DR GeneID; 841486; -.
DR Gramene; AT1G50640.1; AT1G50640.1; AT1G50640. [O80339-1]
DR KEGG; ath:AT1G50640; -.
DR Araport; AT1G50640; -.
DR TAIR; locus:2008021; AT1G50640.
DR eggNOG; ENOG502RYHI; Eukaryota.
DR HOGENOM; CLU_042594_5_0_1; -.
DR InParanoid; O80339; -.
DR OMA; DEDLQCT; -.
DR PhylomeDB; O80339; -.
DR PRO; PR:O80339; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80339; baseline and differential.
DR Genevisible; O80339; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010105; P:negative regulation of ethylene-activated signaling pathway; TAS:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Ethylene signaling pathway; Nucleus;
KW Plant defense; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..225
FT /note="Ethylene-responsive transcription factor 3"
FT /id="PRO_0000112552"
FT DNA_BIND 27..84
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 127..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 199..205
FT /note="EAR-like (transcriptional repression)"
FT COMPBIAS 127..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 2..23
FT /note="RRGRGSSAVAGPTVVAAINGSV -> EKSSCLVRYFRFRRRSCSRLRL (in
FT isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_023535"
FT VAR_SEQ 24..113
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_023536"
SQ SEQUENCE 225 AA; 25216 MW; C709BEABF306776B CRC64;
MRRGRGSSAV AGPTVVAAIN GSVKEIRFRG VRKRPWGRFA AEIRDPWKKA RVWLGTFDSA
EEAARAYDSA ARNLRGPKAK TNFPIDSSSP PPPNLRFNQI RNQNQNQVDP FMDHRLFTDH
QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM KPATTKRYPR TPPVVPEDCH SDCDSSSSVI
DDDDDIASSS RRRNPPFQFD LNFPPLDCVD LFNGADDLHC TDLRL
