ERF78_ARATH
ID ERF78_ARATH Reviewed; 222 AA.
AC O80340; O23107; Q53XI2;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Ethylene-responsive transcription factor 4;
DE Short=AtERF4;
DE AltName: Full=Ethylene-responsive element-binding factor 4;
DE Short=EREBP-4;
DE AltName: Full=Protein RELATED TO APETALA2 5;
GN Name=ERF4; Synonyms=ERF-4, ERF078, RAP2-5; OrderedLocusNames=At3g15210;
GN ORFNames=K7L4.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=9192694; DOI=10.1073/pnas.94.13.7076;
RA Okamuro J.K., Caster B., Villarroel R., Van Montagu M., Jofuku K.D.;
RT "The AP2 domain of APETALA2 defines a large new family of DNA binding
RT proteins in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:7076-7081(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RX PubMed=10715325; DOI=10.2307/3870944;
RA Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.;
RT "Arabidopsis ethylene responsive element binding factors act as
RT transcriptional activators or repressors of GCC box mediated gene
RT expression.";
RL Plant Cell 12:393-404(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION.
RX PubMed=9756931; DOI=10.1074/jbc.273.41.26857;
RA Hao D., Ohme-Takagi M., Sarai A.;
RT "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-
RT responsive element-binding factor (ERF domain) in plant.";
RL J. Biol. Chem. 273:26857-26861(1998).
RN [8]
RP FUNCTION, AND DOMAIN.
RX PubMed=11487705; DOI=10.2307/3871331;
RA Ohta M., Matsui K., Hiratsu K., Shinshi H., Ohme-Takagi M.;
RT "Repression domains of class II ERF transcriptional repressors share an
RT essential motif for active repression.";
RL Plant Cell 13:1959-1968(2001).
RN [9]
RP INDUCTION.
RX PubMed=12805630; DOI=10.1104/pp.102.017814;
RA Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.;
RT "A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene
RT of Arabidopsis.";
RL Plant Physiol. 132:1020-1032(2003).
RN [10]
RP INTERACTION WITH SAP18.
RX PubMed=16429262; DOI=10.1007/s11103-005-3880-9;
RA Song C.-P., Galbraith D.W.;
RT "AtSAP18, an orthologue of human SAP18, is involved in the regulation of
RT salt stress and mediates transcriptional repression in Arabidopsis.";
RL Plant Mol. Biol. 60:241-257(2006).
RN [11]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Acts as a transcriptional repressor. Binds to the GCC-box
CC pathogenesis-related promoter element. Involved in the regulation of
CC gene expression by stress factors and by components of stress signal
CC transduction pathways, and could also regulate other AtERFs.
CC {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:11487705,
CC ECO:0000269|PubMed:9756931}.
CC -!- SUBUNIT: Interacts with SAP18. {ECO:0000269|PubMed:16429262}.
CC -!- INTERACTION:
CC O80340; Q9FIW5: ANAC094; NbExp=3; IntAct=EBI-966009, EBI-25522986;
CC O80340; O64644: At2g45640; NbExp=2; IntAct=EBI-966009, EBI-965964;
CC O80340; Q9LS00: HHO1; NbExp=5; IntAct=EBI-966009, EBI-15203988;
CC O80340; Q8VZS3: HHO2; NbExp=3; IntAct=EBI-966009, EBI-4429217;
CC O80340; Q9FPE8: HHO3; NbExp=3; IntAct=EBI-966009, EBI-2298866;
CC O80340; Q9LSI4: MGH6.1; NbExp=4; IntAct=EBI-966009, EBI-15198743;
CC O80340; O82277: TCP10; NbExp=3; IntAct=EBI-966009, EBI-3133327;
CC O80340; Q9S7W5: TCP13; NbExp=5; IntAct=EBI-966009, EBI-4424877;
CC O80340; Q93Z00: TCP14; NbExp=3; IntAct=EBI-966009, EBI-4424563;
CC O80340; Q9C9L2: TCP15; NbExp=6; IntAct=EBI-966009, EBI-4426144;
CC O80340; Q9M1U4: TCP16; NbExp=5; IntAct=EBI-966009, EBI-15198627;
CC O80340; Q9FTA2: TCP21; NbExp=5; IntAct=EBI-966009, EBI-4426168;
CC O80340; Q9MAH8: TCP3; NbExp=3; IntAct=EBI-966009, EBI-25522447;
CC O80340; Q9LVG2: TOE2; NbExp=3; IntAct=EBI-966009, EBI-4424568;
CC O80340; Q9LDT3: YAB4; NbExp=5; IntAct=EBI-966009, EBI-1115523;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Induced by jasmonate (JA) and by Alternaria brassicicola
CC (locally and systemically), but only slightly by ethylene. Strong
CC induction by wounding, cold or drought stress does not require EIN2,
CC whereas induction by NaCl does. Transcripts accumulate in
CC cycloheximide-treated plants, a protein synthesis inhibitor. Seems to
CC not be influenced by exogenous abscisic acid (ABA), and heat stress.
CC {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:12805630}.
CC -!- DOMAIN: Contains a slightly degenerated ERF-associated amphiphilic
CC repression (EAR) motif, which may be involved in the activity of
CC transcriptional repression. {ECO:0000269|PubMed:11487705}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC49771.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF003098; AAC49771.1; ALT_FRAME; mRNA.
DR EMBL; AB008106; BAA32421.1; -; mRNA.
DR EMBL; AP000413; BAB02150.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75632.1; -; Genomic_DNA.
DR EMBL; AY140030; AAM98171.1; -; mRNA.
DR EMBL; BT010325; AAQ55276.1; -; mRNA.
DR EMBL; AY086232; AAM64308.1; -; mRNA.
DR PIR; T52019; T52019.
DR RefSeq; NP_188139.1; NM_112384.2.
DR AlphaFoldDB; O80340; -.
DR SMR; O80340; -.
DR BioGRID; 6086; 45.
DR IntAct; O80340; 39.
DR STRING; 3702.AT3G15210.1; -.
DR PaxDb; O80340; -.
DR PRIDE; O80340; -.
DR ProteomicsDB; 220567; -.
DR EnsemblPlants; AT3G15210.1; AT3G15210.1; AT3G15210.
DR GeneID; 820752; -.
DR Gramene; AT3G15210.1; AT3G15210.1; AT3G15210.
DR KEGG; ath:AT3G15210; -.
DR Araport; AT3G15210; -.
DR TAIR; locus:2087065; AT3G15210.
DR eggNOG; ENOG502RYHH; Eukaryota.
DR HOGENOM; CLU_042594_5_1_1; -.
DR InParanoid; O80340; -.
DR OMA; MNEIRYR; -.
DR OrthoDB; 1613217at2759; -.
DR PhylomeDB; O80340; -.
DR PRO; PR:O80340; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O80340; baseline and differential.
DR Genevisible; O80340; AT.
DR GO; GO:0016604; C:nuclear body; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; TAS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0071456; P:cellular response to hypoxia; HEP:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009864; P:induced systemic resistance, jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0010105; P:negative regulation of ethylene-activated signaling pathway; TAS:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR GO; GO:0009723; P:response to ethylene; IMP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..222
FT /note="Ethylene-responsive transcription factor 4"
FT /id="PRO_0000112554"
FT DNA_BIND 24..81
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 211..217
FT /note="EAR-like (transcriptional repression)"
SQ SEQUENCE 222 AA; 23741 MW; CEF16ABA5ACFB073 CRC64;
MAKMGLKPDP ATTNQTHNNA KEIRYRGVRK RPWGRYAAEI RDPGKKTRVW LGTFDTAEEA
ARAYDTAARD FRGAKAKTNF PTFLELSDQK VPTGFARSPS QSSTLDCASP PTLVVPSATA
GNVPPQLELS LGGGGGGSCY QIPMSRPVYF LDLMGIGNVG RGQPPPVTSA FRSPVVHVAT
KMACGAQSDS DSSSVVDFEG GMEKRSQLLD LDLNLPPPSE QA
