EHF_MOUSE
ID EHF_MOUSE Reviewed; 300 AA.
AC O70273; Q922E8; Q922T6; Q99K12;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=ETS homologous factor;
DE AltName: Full=ETS domain-containing transcription factor;
GN Name=Ehf {ECO:0000312|MGI:MGI:1270840};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAC40119.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC TISSUE=Pituitary {ECO:0000312|EMBL:AAC40119.1};
RX PubMed=9600089; DOI=10.1006/bbrc.1998.8560;
RA Bochert M.A., Kleinbaum L.A., Sun L.-Y., Burton F.H.;
RT "Molecular cloning and expression of Ehf, a new member of the ets
RT transcription factor/oncoprotein gene family.";
RL Biochem. Biophys. Res. Commun. 246:176-181(1998).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAH05520.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 222-300 (ISOFORMS 1/2).
RC STRAIN=Czech II {ECO:0000312|EMBL:AAH05520.1}, and
RC FVB/N {ECO:0000312|EMBL:AAH08249.1};
RC TISSUE=Mammary gland {ECO:0000312|EMBL:AAH05520.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3] {ECO:0000305}
RP DOMAIN.
RX PubMed=16704374; DOI=10.1042/bj20060375;
RA Choi Y.S., Sinha S.;
RT "Determination of the consensus DNA-binding sequence and a transcriptional
RT activation domain for ESE-2.";
RL Biochem. J. 398:497-507(2006).
CC -!- FUNCTION: Transcriptional activator that may play a role in regulating
CC epithelial cell differentiation and proliferation. May act as a
CC repressor for a specific subset of ETS/AP-1-responsive genes, and as a
CC modulator of the nuclear response to mitogen-activated protein kinase
CC signaling cascades. Binds to DNA sequences containing the consensus
CC nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5
CC expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter
CC (By similarity). {ECO:0000250|UniProtKB:Q9NZC4,
CC ECO:0000269|PubMed:16704374}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NZC4,
CC ECO:0000255|PROSITE-ProRule:PRU00237}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1 {ECO:0000269|PubMed:9600089};
CC IsoId=O70273-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:15489334};
CC IsoId=O70273-2; Sequence=VSP_052191;
CC Name=3 {ECO:0000269|PubMed:15489334};
CC IsoId=O70273-3; Sequence=VSP_052192, VSP_052193;
CC -!- TISSUE SPECIFICITY: Highly expressed in kidney and lung, weakly in
CC skeletal muscle, heart, and liver, and not detected in brain, spleen or
CC testis. {ECO:0000269|PubMed:9600089}.
CC -!- DOMAIN: The PNT domain acts as a transcriptional activator.
CC {ECO:0000269|PubMed:16704374}.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF035527; AAC40119.1; -; mRNA.
DR EMBL; BC005520; AAH05520.1; -; mRNA.
DR EMBL; BC006789; AAH06789.1; -; mRNA.
DR EMBL; BC008249; AAH08249.1; -; mRNA.
DR CCDS; CCDS16475.1; -. [O70273-1]
DR PIR; JW0048; JW0048.
DR RefSeq; NP_031940.1; NM_007914.3. [O70273-1]
DR RefSeq; XP_006498760.1; XM_006498697.2. [O70273-1]
DR RefSeq; XP_006498761.1; XM_006498698.3. [O70273-1]
DR RefSeq; XP_006498762.1; XM_006498699.1. [O70273-1]
DR RefSeq; XP_006498763.1; XM_006498700.3. [O70273-1]
DR RefSeq; XP_006498764.1; XM_006498701.3. [O70273-1]
DR RefSeq; XP_006498765.1; XM_006498702.3. [O70273-1]
DR AlphaFoldDB; O70273; -.
DR SMR; O70273; -.
DR BioGRID; 199409; 1.
DR IntAct; O70273; 1.
DR STRING; 10090.ENSMUSP00000087961; -.
DR PhosphoSitePlus; O70273; -.
DR MaxQB; O70273; -.
DR PaxDb; O70273; -.
DR PeptideAtlas; O70273; -.
DR PRIDE; O70273; -.
DR ProteomicsDB; 277567; -. [O70273-1]
DR ProteomicsDB; 277568; -. [O70273-2]
DR ProteomicsDB; 277569; -. [O70273-3]
DR Antibodypedia; 13027; 140 antibodies from 22 providers.
DR DNASU; 13661; -.
DR Ensembl; ENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350. [O70273-1]
DR Ensembl; ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350. [O70273-1]
DR GeneID; 13661; -.
DR KEGG; mmu:13661; -.
DR UCSC; uc008lio.1; mouse. [O70273-1]
DR CTD; 26298; -.
DR MGI; MGI:1270840; Ehf.
DR VEuPathDB; HostDB:ENSMUSG00000012350; -.
DR eggNOG; KOG3804; Eukaryota.
DR GeneTree; ENSGT00940000159310; -.
DR InParanoid; O70273; -.
DR OMA; SWTDGYP; -.
DR OrthoDB; 837296at2759; -.
DR PhylomeDB; O70273; -.
DR TreeFam; TF318679; -.
DR BioGRID-ORCS; 13661; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Ehf; mouse.
DR PRO; PR:O70273; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; O70273; protein.
DR Bgee; ENSMUSG00000012350; Expressed in lacrimal gland and 141 other tissues.
DR ExpressionAtlas; O70273; baseline and differential.
DR Genevisible; O70273; MM.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0030855; P:epithelial cell differentiation; ISS:UniProtKB.
DR GO; GO:0050673; P:epithelial cell proliferation; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR033071; EHF.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR003118; Pointed_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR PANTHER; PTHR11849:SF171; PTHR11849:SF171; 1.
DR Pfam; PF00178; Ets; 1.
DR Pfam; PF02198; SAM_PNT; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SMART; SM00251; SAM_PNT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR PROSITE; PS51433; PNT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..300
FT /note="ETS homologous factor"
FT /id="PRO_0000257970"
FT DOMAIN 29..115
FT /note="PNT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT DNA_BIND 207..289
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 181..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..197
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 79..93
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_052191"
FT VAR_SEQ 200..211
FT /note="KKHNPRGTHLWE -> YFIIELLLSESR (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_052192"
FT VAR_SEQ 212..300
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_052193"
SQ SEQUENCE 300 AA; 34903 MW; 91D9477EFB874747 CRC64;
MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW TKYQVWEWLQ
HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA GQLLYSNLQH LKWNGQCSSD
LFQSAHNVIV KTEQTDPSIM NTWKEENYLY DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP
VAESPDMKKE QDHPVKSHTK KHNPRGTHLW EFIRDILLSP DKNPGLIKWE DRSEGIFRFL
KSEAVAQLWG KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN
