ID   3HIDH_CAEEL             Reviewed;         299 AA.
AC   Q9XTI0;
DT   24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial;
DE            Short=HIBADH;
DE            EC=1.1.1.31;
GN   ORFNames=B0250.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-
CC         oxopropanoate + H(+) + NADH; Xref=Rhea:RHEA:17681, ChEBI:CHEBI:11805,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57700,
CC         ChEBI:CHEBI:57945; EC=1.1.1.31;
CC   -!- PATHWAY: Amino-acid degradation; L-valine degradation.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HIBADH-related family. 3-hydroxyisobutyrate
CC       dehydrogenase subfamily. {ECO:0000305}.
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DR   EMBL; Z81453; CAB03798.1; -; Genomic_DNA.
DR   EMBL; AL031630; CAB03798.1; JOINED; Genomic_DNA.
DR   PIR; T18682; T18682.
DR   RefSeq; NP_507943.1; NM_075542.6.
DR   AlphaFoldDB; Q9XTI0; -.
DR   SMR; Q9XTI0; -.
DR   BioGRID; 45301; 11.
DR   STRING; 6239.B0250.5; -.
DR   iPTMnet; Q9XTI0; -.
DR   EPD; Q9XTI0; -.
DR   PaxDb; Q9XTI0; -.
DR   PeptideAtlas; Q9XTI0; -.
DR   EnsemblMetazoa; B0250.5.1; B0250.5.1; WBGene00007122.
DR   GeneID; 180344; -.
DR   UCSC; B0250.5; c. elegans.
DR   CTD; 180344; -.
DR   WormBase; B0250.5; CE18481; WBGene00007122; -.
DR   eggNOG; KOG0409; Eukaryota.
DR   GeneTree; ENSGT00940000173744; -.
DR   HOGENOM; CLU_035117_6_0_1; -.
DR   InParanoid; Q9XTI0; -.
DR   OMA; WSSEVNN; -.
DR   OrthoDB; 812358at2759; -.
DR   PhylomeDB; Q9XTI0; -.
DR   Reactome; R-CEL-70895; Branched-chain amino acid catabolism.
DR   UniPathway; UPA00362; -.
DR   PRO; PR:Q9XTI0; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00007122; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR   GO; GO:0006574; P:valine catabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR006115; 6PGDH_NADP-bd.
DR   InterPro; IPR011548; HIBADH.
DR   InterPro; IPR015815; HIBADH-related.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR029154; NADP-bd.
DR   Pfam; PF14833; NAD_binding_11; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PIRSF; PIRSF000103; HIBADH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01692; HIBADH; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE   3: Inferred from homology;
KW   Branched-chain amino acid catabolism; Mitochondrion; NAD; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..299
FT                   /note="Probable 3-hydroxyisobutyrate dehydrogenase,
FT                   mitochondrial"
FT                   /id="PRO_0000173055"
FT   ACT_SITE        172
FT                   /evidence="ECO:0000250"
FT   BINDING         2..30
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         96
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         247
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   299 AA;  31217 MW;  F30B423A597CABF0 CRC64;
     MSLTGFIGLG NMGGHMARNL IKNGKKLIVY DVNKAVVQEF KAEGCEVAAH PADIAAASKE
     IITVLPSSPH VKAVYQGEAG IFKTIQPGTL CMDSSTIDQI VSLEVAQAAA LLKAEYIDAP
     ISGGVTGAQQ ATLTFMVGAG NDATFKRAEA VLSLMGKNIV NLGAVGNGTA AKICNNMLLG
     IQMVAVAETM NLGISMGLDA KALAGIVNTS SGRCWSSDTY NPVPGVIENI PSCRGYAGGF
     GTTLMAKDLS LAQNASTNTQ APTPMGSLAH QIYRILARDP QYQAKDFGVV YQFLKKQNS