DPO3_THEMA
ID DPO3_THEMA Reviewed; 1367 AA.
AC Q9ZHF6;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=TM_0576;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9826752; DOI=10.1093/nar/26.23.5300;
RA Huang Y.P., Ito J.;
RT "The hyperthermophilic bacterium Thermotoga maritima has two different
RT classes of family C DNA polymerases: evolutionary implications.";
RL Nucleic Acids Res. 26:5300-5309(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AF065313; AAC80438.1; -; Genomic_DNA.
DR EMBL; AE000512; AAD35661.1; -; Genomic_DNA.
DR PIR; C72360; C72360.
DR RefSeq; NP_228386.1; NC_000853.1.
DR RefSeq; WP_004081285.1; NZ_CP011107.1.
DR PDB; 2P1J; X-ray; 2.50 A; A/B=347-522.
DR PDBsum; 2P1J; -.
DR AlphaFoldDB; Q9ZHF6; -.
DR SMR; Q9ZHF6; -.
DR STRING; 243274.THEMA_01790; -.
DR EnsemblBacteria; AAD35661; AAD35661; TM_0576.
DR KEGG; tma:TM0576; -.
DR eggNOG; COG2176; Bacteria.
DR InParanoid; Q9ZHF6; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR EvolutionaryTrace; Q9ZHF6; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 2.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW Exonuclease; Hydrolase; Nuclease; Nucleotidyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..1367
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204605"
FT DOMAIN 358..513
FT /note="Exonuclease"
FT STRAND 358..366
FT /evidence="ECO:0007829|PDB:2P1J"
FT TURN 370..372
FT /evidence="ECO:0007829|PDB:2P1J"
FT STRAND 375..384
FT /evidence="ECO:0007829|PDB:2P1J"
FT STRAND 387..395
FT /evidence="ECO:0007829|PDB:2P1J"
FT HELIX 404..410
FT /evidence="ECO:0007829|PDB:2P1J"
FT HELIX 414..417
FT /evidence="ECO:0007829|PDB:2P1J"
FT HELIX 423..433
FT /evidence="ECO:0007829|PDB:2P1J"
FT STRAND 434..436
FT /evidence="ECO:0007829|PDB:2P1J"
FT STRAND 438..441
FT /evidence="ECO:0007829|PDB:2P1J"
FT HELIX 444..459
FT /evidence="ECO:0007829|PDB:2P1J"
FT STRAND 467..469
FT /evidence="ECO:0007829|PDB:2P1J"
FT HELIX 470..477
FT /evidence="ECO:0007829|PDB:2P1J"
FT HELIX 485..491
FT /evidence="ECO:0007829|PDB:2P1J"
FT HELIX 501..516
FT /evidence="ECO:0007829|PDB:2P1J"
SQ SEQUENCE 1367 AA; 155363 MW; EE5916FA70591F84 CRC64;
MKKIENLKWK NVSFKSLEID PDAGVVLVSV EKFSEEIEDL VRLLEKKTRF RVIVNGVQKS
NGDLRGKILS LLNGNVPYIK DVVFEGNRLI LKVLGDFARD RIASKLRSTK KQLDELLPPG
TEIMLEVVEP PEDLLKKEVP QPEKREEPKG EELKIEDENH IFGQKPRKIV FTPSKIFEYN
KKTSVKGKIF KIEKIEGKRT VLLIYLTDGE DSLICKVFND VEKVEGKVSV GDVIVATGDL
LLENGEPTLY VKGITKLPEA KRMDKSPVKR VELHAHTKFS DQDAITDVNE YVKRAKEWGF
PAIALTDHGN VQAIPYFYDA AKEAGIKPIF GIEAYLVSDV EPVIRNLSDD STFGDATFVV
LDFETTGLDP QVDEIIEIGA VKIQGGQIVD EYHTLIKPSR EISRKSSEIT GITQEMLENK
RSIEEVLPEF LGFLEDSIIV AHNANFDYRF LRLWIKKVMG LDWERPYIDT LALAKSLLKL
RSYSLDSVVE KLGLGPFRHH RALDDARVTA QVFLRFVEMM KKIGITKLSE MEKLKDTIDY
TALKPFHCTI LVQNKKGLKN LYKLVSDSYI KYFYGVPRIL KSELIENREG LLVGSACISG
ELGRAALEGA SDSELEEIAK FYDYIEVMPL DVIAEDEEDL DRERLKEVYR KLYRIAKKLN
KFVVMTGDVH FLDPEDARGR AALLAPQGNR NFENQPALYL RTTEEMLEKA IEIFEDEEIA
REVVIENPNR IADMIEEVQP LEKKLHPPII ENADEIVRNL TMKRAYEIYG DPLPEIVQKR
VEKELNAIIN HGYAVLYLIA QELVQKSMSD GYVVGSRGSV GSSLVANLLG ITEVNPLPPH
YRCPECKYFE VVEDDRYGAG YDLPNKNCPR CGAPLRKDGH GIPFETFMGF EGDKVPDIDL
NFSGEYQERA HRFVEELFGK DHVYRAGTIN TIAERSAVGY VRSYEEKTGK KLRKAEMERL
VSMITGVKRT TGQHPGGLMI IPKDKEVYDF TPIQYPANDR NAGVFTTHFA YETIHDDLVK
IDALGHDDPT FIKMLKDLTG IDPMTIPMDD PDTLAIFSSV KPLGVDPVEL ESDVGTYGIP
EFGTEFVRGM LVETRPKSFA ELVRISGLSH GTDVWLNNAR DWINLGYAKL SEVISCRDDI
MNFLIHKGME PSLAFKIMEN VRKGKGITEE MESEMRRLKV PEWFIESCKR IKYLFPKAHA
VAYVSMAFRI AYFKVHYPLQ FYAAYFTIKG DQFDPVLVLR GKEAIKRRLR ELKAMPAKDA
QKKNEVSVLE VALEMILRGF SFLPPDIFKS DAKKFLIEGN SLRIPFNKLP GLGDSVAESI
IRAREEKPFT SVEDLMKRTK VNKNHIELMK SLGVLGDLPE TEQFTLF
