ID   DPO3A_STRPQ             Reviewed;        1036 AA.
AC   P0DA75; Q8K7A1;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=SPs1113;
OS   Streptococcus pyogenes serotype M3 (strain SSI-1).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=193567;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SSI-1;
RX   PubMed=12799345; DOI=10.1101/gr.1096703;
RA   Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y.,
RA   Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H.,
RA   Hattori M., Hamada S.;
RT   "Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-
RT   scale genomic rearrangement in invasive strains and new insights into phage
RT   evolution.";
RL   Genome Res. 13:1042-1055(2003).
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BA000034; BAC64208.1; -; Genomic_DNA.
DR   RefSeq; WP_011054545.1; NC_004606.1.
DR   AlphaFoldDB; P0DA75; -.
DR   SMR; P0DA75; -.
DR   KEGG; sps:SPs1113; -.
DR   HOGENOM; CLU_001600_0_0_9; -.
DR   OMA; NECRRMG; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 2.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1036
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000411325"
SQ   SEQUENCE   1036 AA;  119153 MW;  9DD548F3048D3A39 CRC64;
     MFAQLDTKTV YSFMDSLIDL NHYFERAKQF GYHTIGIMDK DNLYGAYHFI KGCQKNGLQP
     VLGLEVEILY QERQVLLNLI AQNTQGYHQL LKISTAKMSG KLHMDYLCQH LEGIAVIIPS
     KGWSDTLVVP FDYYIGVDQY TDLSHMDSKR QLIPLRTVRY FAQDDMETLH MLHAIRDNLS
     LAETPVVESD QELTDCQQLT TFYQTHCPQA LQNLEDLVSG IYYDFDTNLK LPHFNRDKSA
     KQELQELTEA GLKEKGLWKE PYQSRLLHEL VIISDMGFDD YFLIVWDLLR FGRSKGYYMG
     MGRGSAAGSL VAYALNITGI DPVQHDLLFE RFLNKERYSM PDIDIDLPDI YRSEFLRYVR
     NRYGSDHSAQ IVTFSTFGPK QAIRDVFKRF GVPEYELTNL TKKIGFKDSL ATVYEKSISF
     RQVINSRTEF QKAFAIAKRI EGNPRQTSIH AAGIVMSDDT LTNHIPLKSG DDMMITQYDA
     HAIEANGLLK MDFLGLRNLT FVQKMQEKVA KDYGCQIDIA AIDLEDPQTL ALFAKGDTKG
     IFQFEQNGAI NLLKRIKPQR FEEIVATTSL NRPGASDYTT NFIKRREGQE KIDLIDPVIA
     PILEPTYGIM LYQEQVMQIA QIYAGFTLGK ADLLRRAMSK KNLQEMQKME EDFIASAKHL
     GRAEETARGL FKRMEKFAGY GFNRSHAFAY SALAFQLAYF KAHYPAVFYD IMMNYSSSDY
     ITDALESDFQ VAQVTINSIP YTDKIEASKI YMGLKNIKGL PRDFAYWIIE QRPFNSVEDF
     LTRTPEKYQK KVFLEPLIKI GLFDCFEPNR KKILDNLDGL LVFVNELGSL FSDSSFSWVD
     AKDYSATEKY SLEQEIVGVG MSKHPLIDIA EKSTQTFTPI SQLVKESEAV VLIQIDSIRI
     IRTKTSGQQM AFLSVNDTKK KLDVTLFPQE YAIYKDQLIE GEFYYLKGRI KERDHRLQMV
     CQQVQMAISQ KYWLLVENHQ FDSQISEILG AFPGTTPVVI HYQKNKETIA LTKIQVHVTE
     NLKEKLRPFV LKTVFR