DMD4_CAEEL
ID DMD4_CAEEL Reviewed; 260 AA.
AC Q18248;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Doublesex- and mab-3-related transcription factor dmd-4 {ECO:0000305};
GN Name=dmd-4 {ECO:0000312|WormBase:C27C12.6};
GN ORFNames=C27C12.6 {ECO:0000312|WormBase:C27C12.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH UBIQUITIN, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, DOMAIN, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF LEU-174.
RX PubMed=33021200; DOI=10.7554/elife.59614;
RA Bayer E.A., Stecky R.C., Neal L., Katsamba P.S., Ahlsen G., Balaji V.,
RA Hoppe T., Shapiro L., Oren-Suissa M., Hobert O.;
RT "Ubiquitin-dependent regulation of a conserved DMRT protein controls
RT sexually dimorphic synaptic connectivity and behavior.";
RL Elife 9:0-0(2020).
CC -!- FUNCTION: Probable transcription factor, involved in regulation of
CC sexually dimorphic synaptic connectivity and behavior
CC (PubMed:33021200). Promotes pruning of phasmid neuron synaptic
CC connections in hermaphrodite animals during sexual maturation
CC (PubMed:33021200). {ECO:0000269|PubMed:33021200}.
CC -!- SUBUNIT: Monomer (PubMed:33021200). May interact with ubiquitin; the
CC interaction may stabilize dmd-4, especially in neurons
CC (PubMed:33021200). {ECO:0000269|PubMed:33021200}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070}.
CC -!- TISSUE SPECIFICITY: Expressed in PHA and PHB phasmid sensory neuron
CC pairs of adult hermaphrodites, but not adult males (PubMed:33021200).
CC Expressed in sexually non-dimorphic manner in some head sensory neurons
CC (AFD, AWB, AWC, ASE, ASG, ASH, BAG), a single pharyngeal neuron (I5)
CC and a number of MS blastomere derived cells - the head mesodermal cell,
CC (hmc), a single pharyngeal muscle (pm8), a pharyngeal gland cell pair
CC (g2L/R) and four of the six pharyngeal intestinal valve cells
CC (PubMed:33021200). {ECO:0000269|PubMed:33021200}.
CC -!- DEVELOPMENTAL STAGE: First expressed in mid-embryonic development, in
CC the head mesodermal cell, (hmc), a single pharyngeal muscle (pm8), a
CC pharyngeal gland cell pair (g2L/R) and four of the six pharyngeal
CC intestinal valve cells (PubMed:33021200). Around hatching, expressed in
CC some head and tail sensory neurons, including the phasmid neurons PHA
CC and PHB (PubMed:33021200). During larval development, expression is the
CC same in both sexes until the larval L4 stage to adult molt, when
CC expression in PHA and PHB in the male disappears, but continues in the
CC hermaphrodite (PubMed:33021200). {ECO:0000269|PubMed:33021200}.
CC -!- DOMAIN: DMA domain may interact with ubiquitin.
CC {ECO:0000269|PubMed:33021200}.
CC -!- DISRUPTION PHENOTYPE: Causes embryonic lethality (PubMed:33021200).
CC Abnormal anterior intestinal function (PubMed:33021200).
CC {ECO:0000269|PubMed:33021200}.
CC -!- SIMILARITY: Belongs to the DMRT family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284606; CAA93739.2; -; Genomic_DNA.
DR PIR; E89714; E89714.
DR PIR; T19516; T19516.
DR RefSeq; NP_510466.1; NM_078065.3.
DR AlphaFoldDB; Q18248; -.
DR SMR; Q18248; -.
DR IntAct; Q18248; 2.
DR STRING; 6239.C27C12.6; -.
DR PaxDb; Q18248; -.
DR EnsemblMetazoa; C27C12.6.1; C27C12.6.1; WBGene00007776.
DR GeneID; 181581; -.
DR KEGG; cel:CELE_C27C12.6; -.
DR UCSC; C27C12.6; c. elegans.
DR CTD; 181581; -.
DR WormBase; C27C12.6; CE23560; WBGene00007776; dmd-4.
DR eggNOG; KOG3815; Eukaryota.
DR GeneTree; ENSGT00940000168442; -.
DR HOGENOM; CLU_1086800_0_0_1; -.
DR InParanoid; Q18248; -.
DR OMA; HCKYKEC; -.
DR OrthoDB; 1341962at2759; -.
DR PhylomeDB; Q18248; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00007776; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043130; F:ubiquitin binding; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IBA:GO_Central.
DR GO; GO:0098883; P:synapse pruning; IMP:UniProtKB.
DR Gene3D; 4.10.1040.10; -; 1.
DR InterPro; IPR001275; DM_DNA-bd.
DR InterPro; IPR036407; DM_DNA-bd_sf.
DR InterPro; IPR005173; DMA.
DR InterPro; IPR026607; DMRT.
DR PANTHER; PTHR12322; PTHR12322; 1.
DR Pfam; PF00751; DM; 1.
DR Pfam; PF03474; DMA; 1.
DR SMART; SM00301; DM; 1.
DR SUPFAM; SSF82927; SSF82927; 1.
DR PROSITE; PS40000; DM_1; 1.
DR PROSITE; PS50809; DM_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..260
FT /note="Doublesex- and mab-3-related transcription factor
FT dmd-4"
FT /id="PRO_0000453458"
FT DOMAIN 144..180
FT /note="DMA"
FT /evidence="ECO:0000255"
FT DNA_BIND 22..69
FT /note="DM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT REGION 232..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 174
FT /note="L->R: In ot990; results in defective pruning on the
FT PHB to AVG synaptic connection in hermaphrodites. May
FT affect binding to ubiquitin."
FT /evidence="ECO:0000269|PubMed:33021200"
SQ SEQUENCE 260 AA; 29365 MW; 54933AB8AF3CED7D CRC64;
MMIGNLHVFP NGRIERERKP KCARCRNHGL VSWLKGHKRH CKYKECACEK CNLIAERQRV
MAAQVALKRR QATEDAIALG LRVVAGQAID RLPQGPVWNT AGGEDEDMDY LDEEIEEKPP
TPVSEELVPL KRKKVEETYE LSSFSPIELL MTLFCEHEKH VLELVLEACH GNVLQAIEHF
ANVRRVKNIN QMKLFAAATR MDHVFPNMTN FILPKQSFLI DSLLEQPTFP VSSQASTSTK
TCDSSSVDSI SPNSSTFESS
