ID   DLHH_AZOBR              Reviewed;         231 AA.
AC   Q43914;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
OS   Azospirillum brasilense.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Azospirillaceae; Azospirillum.
OX   NCBI_TaxID=192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29145 / DSM 1690 / IMET 11303 / Sp7;
RX   PubMed=8138139; DOI=10.1111/j.1574-6968.1994.tb06650.x;
RA   Zimmer W., Hundeshagen B.;
RT   "Identification and sequencing of pyrG, the CTP synthetase gene of
RT   Azospirillum brasilense Sp7.";
RL   FEMS Microbiol. Lett. 115:273-278(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; X67216; CAA47657.1; -; Genomic_DNA.
DR   PIR; I39497; I39497.
DR   AlphaFoldDB; Q43914; -.
DR   SMR; Q43914; -.
DR   ESTHER; azobr-dlhh; Dienelactone_hydrolase.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 2.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..231
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000161577"
FT   ACT_SITE        169
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        200
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   231 AA;  24866 MW;  B7B76E567F9E0D1E CRC64;
     MTDISIPAGD DGSFSAYVAK PAGGGPAPGL VVIQEIFGVN QVMRDLCDAF AAQGWLAVCP
     DLFWRQEPGV QITDKTQEEW NRAFALMNGM DQDKRWTTSR PPCRGCARIR IAPAKRVGRL
     LLGRRLAFMM AARSDSDANV SYYGVGLDGL VGEAASITKP LLMHIAEKDQ FVPAEAREKV
     LAAVKGNPNV TAHVYPGVDH AFARAGGAHF EPEAAELANG RTAAFFKQHL G