ID   DJ27B_XENLA             Reviewed;         276 AA.
AC   Q5M7D1; Q6IML2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=DnaJ homolog subfamily C member 27-B;
DE   AltName: Full=Rab and DnaJ domain-containing protein 2;
DE   AltName: Full=Rab and DnaJ domain-containing protein B;
GN   Name=dnajc27-b; Synonyms=rbj2, rjb-b;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 30-178.
RA   Clifton S., Johnson S.L., Blumberg B., Song J., Hillier L., Pape D.,
RA   Martin J., Wylie T., Underwood K., Theising B., Bowers Y., Person B.,
RA   Gibbons M., Harvey N., Ritter E., Jackson Y., McCann R., Waterston R.,
RA   Wilson R.;
RT   "WashU-NCI Xenopus EST project.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=14980719; DOI=10.1016/j.gene.2003.11.010;
RA   Nepomuceno-Silva J.L., de Melo L.D., Mendonca S.M., Paixao J.C.,
RA   Lopes U.G.;
RT   "RJLs: a new family of Ras-related GTP-binding proteins.";
RL   Gene 327:221-232(2004).
CC   -!- FUNCTION: GTPase possibly involved in regulation of the MEK/ERK
CC       pathway. {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; BC088700; AAH88700.1; -; mRNA.
DR   EMBL; BE025902; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BK001291; DAA01330.1; -; mRNA.
DR   RefSeq; NP_001088891.1; NM_001095422.1.
DR   AlphaFoldDB; Q5M7D1; -.
DR   SMR; Q5M7D1; -.
DR   DNASU; 496237; -.
DR   GeneID; 496237; -.
DR   KEGG; xla:496237; -.
DR   CTD; 496237; -.
DR   Xenbase; XB-GENE-490424; dnajc27.S.
DR   OMA; MGNAETG; -.
DR   OrthoDB; 1288652at2759; -.
DR   Proteomes; UP000186698; Chromosome 5S.
DR   Bgee; 496237; Expressed in brain and 19 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   2: Evidence at transcript level;
KW   GTP-binding; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..276
FT                   /note="DnaJ homolog subfamily C member 27-B"
FT                   /id="PRO_0000332982"
FT   DOMAIN          220..276
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   BINDING         23..30
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         71..75
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         137..140
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        78
FT                   /note="F -> S (in Ref. 2; BE025902)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   276 AA;  31278 MW;  38AC57E22D2D1F25 CRC64;
     MESHLQKRKD SRKPLRIKVI SMGNAEVGKS CIIKRYCEKR FVPKYQATIG IDYGVTKVQI
     KDREIKVNIF DMAGHPFFYE VRNEFYKDTQ GVILVYDVGQ KESFESLDAW LAEMKQELGP
     QINIDNLDNI VFAVCANKID STKHRCVDES EGRLWSESKG FLYFETSAQS GEGINEMFQA
     FYSSIVDLCD NGGKRPVSAI NIGFTKEQAD SIRRIRNSKD SWDMLGVKPG ATRDEVNKAY
     RKLAVLLHPD KCVAPGSEDA FKAVVNARTA LLKNIK