DHX15_DICDI
ID DHX15_DICDI Reviewed; 727 AA.
AC Q54NJ4;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Putative ATP-dependent RNA helicase DHX15;
DE EC=3.6.4.13;
DE AltName: Full=DEAH box protein 15;
GN Name=dhx15; Synonyms=prp43; ORFNames=DDB_G0285213;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Pre-mRNA processing factor involved in disassembly of
CC spliceosomes after the release of mature mRNA.
CC {ECO:0000250|UniProtKB:O43143}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000250|UniProtKB:O43143};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43143}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC DDX15/PRP43 sub-subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000076; EAL64818.1; -; Genomic_DNA.
DR RefSeq; XP_638323.1; XM_633231.1.
DR AlphaFoldDB; Q54NJ4; -.
DR SMR; Q54NJ4; -.
DR STRING; 44689.DDB0233403; -.
DR PaxDb; Q54NJ4; -.
DR PRIDE; Q54NJ4; -.
DR EnsemblProtists; EAL64818; EAL64818; DDB_G0285213.
DR GeneID; 8624993; -.
DR KEGG; ddi:DDB_G0285213; -.
DR dictyBase; DDB_G0285213; dhx15.
DR eggNOG; KOG0925; Eukaryota.
DR HOGENOM; CLU_001832_7_2_1; -.
DR InParanoid; Q54NJ4; -.
DR OMA; DHDLKRY; -.
DR PhylomeDB; Q54NJ4; -.
DR Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR PRO; PR:Q54NJ4; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; ISS:dictyBase.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd17973; DEXHc_DHX15; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044756; DHX15_DEXHc.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; mRNA processing; mRNA splicing;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..727
FT /note="Putative ATP-dependent RNA helicase DHX15"
FT /id="PRO_0000330940"
FT DOMAIN 87..251
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 273..445
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 198..201
FT /note="DEAH box"
FT COMPBIAS 1..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 100..107
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 727 AA; 82858 MW; 153EA093D51E89FB CRC64;
MSKRKHESSD SNKKAMKKQQ NKIEEEEEEI TNTTTTTTTT NNNYNNIKSN LTIDQWIPKK
ETFSKRYYEI LEKRKELPVW KQKEDFIKVI KENQVVILVG ETGSGKTTQI PQFVVDAGLI
RPGKMVGVTQ PRRVAAISVA KRVSEEMDFE LGEEVGYSIR FEELSSARTF MKYLTDGMLL
RESMSDPTLN KYDVIILDEA HERTLSTDIL FGLIKDILKR RKDLKLIVMS ATLEAGKFQK
YFENAPLIKV PGRLHPVEIF YTEEAAKDYL ESAVRTVIDI HTNEGTGDIL VFLTGEEEIE
DTCAKIQRET RERGLPPMKT LPLYSSLPIY QQSKIFDTCK ERKCIVSTNI AETSLTIDGI
VFVVDPGFSK QKTYNPRSRV ESLLVAPISK ASANQRAGRA GRTRPGKCFR LYTEKAFKEL
MIQQTHPEIL RSNLASVVLQ LLKLGVVDLV HFDFMDPPVP DTLIRALEVL HYLGALDDEG
QLTEIGSIMS EFPLDPQLSK MLIVSAERSC SNEILTIAAM LSAPNCFMRP KDNRIEADSA
KKSFDHFDGD HLTMLNVYHS FKKNGEDPTW CYDNFLNHRA IKQADSVRSQ LARILTRFKL
PLVSGDVNSK FYYENIKKCI AAGFFMQVAK CEKKNIYFTL GDEQSVIFHP STGLTRRPEF
CIYNEFVLTS ENYIRTITDV KFDWLLELAP SYFKQKSFPK KTKETIQRAQ RLYSGSSSGS
SSGSNKK
