DHRS9_BOVIN
ID DHRS9_BOVIN Reviewed; 319 AA.
AC Q8HYR6;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Dehydrogenase/reductase SDR family member 9;
DE EC=1.1.1.209 {ECO:0000250|UniProtKB:Q9BPW9};
DE EC=1.1.1.53 {ECO:0000250|UniProtKB:Q9BPW9};
DE AltName: Full=3-alpha hydroxysteroid dehydrogenase;
DE Short=3-alpha-HSD;
DE AltName: Full=Retinol dehydrogenase;
DE EC=1.1.1.105 {ECO:0000250|UniProtKB:Q9BPW9};
DE AltName: Full=Short-chain dehydrogenase/reductase retSDR8;
DE Flags: Precursor;
GN Name=DHRS9;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Eye;
RX PubMed=10800688; DOI=10.1016/s0076-6879(00)16736-9;
RA Haeseleer F., Palczewski K.;
RT "Short-chain dehydrogenases/reductases in retina.";
RL Methods Enzymol. 316:372-383(2000).
CC -!- FUNCTION: 3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-
CC tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and
CC 3-alpha-androstanediol to dihydroxyprogesterone. Also plays a role in
CC the biosynthesis of retinoic acid from retinaldehyde. Can utilize both
CC NADH and NADPH. {ECO:0000250|UniProtKB:Q8VD48,
CC ECO:0000250|UniProtKB:Q9BPW9}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3beta-hydroxy-5alpha-pregnane-20-one + NAD(+) = 5alpha-
CC pregnane-3,20-dione + H(+) + NADH; Xref=Rhea:RHEA:41988,
CC ChEBI:CHEBI:11909, ChEBI:CHEBI:15378, ChEBI:CHEBI:28952,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC Evidence={ECO:0000250|UniProtKB:Q9BPW9};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17beta-hydroxy-5alpha-androstan-3-one + NAD(+) = 5alpha-
CC androstan-3,17-dione + H(+) + NADH; Xref=Rhea:RHEA:41992,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15994, ChEBI:CHEBI:16330,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC Evidence={ECO:0000250|UniProtKB:Q9BPW9};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3alpha-hydroxy-5alpha-androstan-17-one + NAD(+) = 5alpha-
CC androstan-3,17-dione + H(+) + NADH; Xref=Rhea:RHEA:20381,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15994, ChEBI:CHEBI:16032,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.209;
CC Evidence={ECO:0000250|UniProtKB:Q9BPW9};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5alpha-androstane-3alpha,17beta-diol + NAD(+) = 17beta-
CC hydroxy-5alpha-androstan-3-one + H(+) + NADH; Xref=Rhea:RHEA:42004,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16330, ChEBI:CHEBI:36713,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.53;
CC Evidence={ECO:0000250|UniProtKB:Q9BPW9};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinol + NAD(+) = all-trans-retinal + H(+) + NADH;
CC Xref=Rhea:RHEA:21284, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336,
CC ChEBI:CHEBI:17898, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.1.1.105; Evidence={ECO:0000250|UniProtKB:Q9BPW9};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3alpha-hydroxy-5alpha-pregnan-20-one + NAD(+) = 5alpha-
CC pregnane-3,20-dione + H(+) + NADH; Xref=Rhea:RHEA:41980,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28952, ChEBI:CHEBI:50169,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC Evidence={ECO:0000250|UniProtKB:Q9BPW9};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Microsome membrane
CC {ECO:0000250|UniProtKB:Q9BPW9}. Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9BPW9}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF362071; AAN75756.1; -; mRNA.
DR RefSeq; NP_777158.1; NM_174733.2.
DR AlphaFoldDB; Q8HYR6; -.
DR SMR; Q8HYR6; -.
DR STRING; 9913.ENSBTAP00000055524; -.
DR PaxDb; Q8HYR6; -.
DR PRIDE; Q8HYR6; -.
DR Ensembl; ENSBTAT00000005986; ENSBTAP00000005986; ENSBTAG00000004557.
DR GeneID; 282851; -.
DR KEGG; bta:282851; -.
DR CTD; 10170; -.
DR VEuPathDB; HostDB:ENSBTAG00000004557; -.
DR VGNC; VGNC:28046; DHRS9.
DR eggNOG; KOG1610; Eukaryota.
DR GeneTree; ENSGT00940000158665; -.
DR HOGENOM; CLU_010194_2_0_1; -.
DR InParanoid; Q8HYR6; -.
DR OMA; PQTHYIA; -.
DR OrthoDB; 1313182at2759; -.
DR Reactome; R-BTA-2453902; The canonical retinoid cycle in rods (twilight vision).
DR Reactome; R-BTA-5365859; RA biosynthesis pathway.
DR Proteomes; UP000009136; Chromosome 2.
DR Bgee; ENSBTAG00000004557; Expressed in milk and 49 other tissues.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0004022; F:alcohol dehydrogenase (NAD+) activity; ISS:UniProtKB.
DR GO; GO:0047044; F:androstan-3-alpha,17-beta-diol dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0047023; F:androsterone dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0004745; F:NAD-retinol dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016229; F:steroid dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0047035; F:testosterone dehydrogenase (NAD+) activity; ISS:UniProtKB.
DR GO; GO:0042904; P:9-cis-retinoic acid biosynthetic process; ISS:UniProtKB.
DR GO; GO:0008209; P:androgen metabolic process; IEA:Ensembl.
DR GO; GO:0042448; P:progesterone metabolic process; IEA:Ensembl.
DR GO; GO:0042572; P:retinol metabolic process; IBA:GO_Central.
DR GO; GO:0008202; P:steroid metabolic process; IBA:GO_Central.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Lipid metabolism; Membrane; Microsome; NAD; NADP;
KW Oxidoreductase; Reference proteome; Signal; Steroid metabolism.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..319
FT /note="Dehydrogenase/reductase SDR family member 9"
FT /id="PRO_0000042616"
FT ACT_SITE 176
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9BPW9"
FT BINDING 34..58
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 83
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 164
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 180
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 319 AA; 35388 MW; 7AA3847670F57CAD CRC64;
MLFWLLVLLI LCGFLWNYKR QLKIANITDK YIFITGCDTG FGNLAARTFD KKGFHVIAAC
LTESGSTALK AETSERLHTV LLDVTDPENV KRAAQWVKNQ VGEKGLWGLI NNAGILGVLA
PNDWLTVEDY REPVEVNLFG LISVTLNMLP LVKKARGRII NVSSIGGRLA FGGGGYSPSK
YAVEGFNDSL RRDMKAFGVH VACIEPGLFK TNLSDPEKTA EKKLAIWKHL SPDIKQQYGE
SYIEKSLEQL KGTASFVNVD LSLVVECMDH ALTSLFPKTR YAAGKDAKTF WIPLSHMPAV
LQDFLLLKQK VELANPRAV
