ID   DHNO_AGRVI              Reviewed;         412 AA.
AC   Q44524;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=D-nopaline dehydrogenase;
DE            EC=1.5.1.19;
DE   AltName: Full=Nopaline synthase;
GN   Name=nos;
OS   Agrobacterium vitis (Rhizobium vitis).
OG   Plasmid pTiAB4.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=373;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AB4;
RX   PubMed=7808399; DOI=10.1007/bf00302262;
RA   Otten L., de Ruffray P.;
RT   "Agrobacterium vitis nopaline Ti plasmid pTiAB4: relationship to other Ti
RT   plasmids and T-DNA structure.";
RL   Mol. Gen. Genet. 245:493-505(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-nopaline + H2O + NADP(+) = 2-oxoglutarate + H(+) + L-
CC         arginine + NADPH; Xref=Rhea:RHEA:19637, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16810, ChEBI:CHEBI:32682,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58074, ChEBI:CHEBI:58349; EC=1.5.1.19;
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lysopine/nopaline/octopine/opine/vitopine
CC       dehydrogenases family. {ECO:0000305}.
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DR   EMBL; X77327; CAA54543.1; -; Genomic_DNA.
DR   PIR; S41895; S41895.
DR   AlphaFoldDB; Q44524; -.
DR   SMR; Q44524; -.
DR   GO; GO:0047829; F:D-nopaline dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR011128; G3P_DH_NAD-dep_N.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR003421; Opine_DH.
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR   Pfam; PF02317; Octopine_DH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; NADP; Oxidoreductase; Plasmid.
FT   CHAIN           1..412
FT                   /note="D-nopaline dehydrogenase"
FT                   /id="PRO_0000179984"
SQ   SEQUENCE   412 AA;  45279 MW;  CEBD6858EA2AE395 CRC64;
     MRLASSSTSL PVPAAGHHPL PLTVGVLGSG HAGTALAAWF ASRHVSTALW APADHPGSIS
     AIKANEGVVT TEGVINGSFT VSASDDLLAV IRSSHLLIIV TRADVHDSFV SELANFNGEL
     AEKDILVVCG HGFSMKYERQ LQCKRILETD NSPTTSKLSD KKKCKVNIKE MKASFGLSCF
     PIHRNDVGVI DLPEDIKIIF AQLFTARIIP IPPLQVLFFS NYITHAVAAV MNIGSVRDPV
     NSLTKRAEQW LLELDERTPR AETGFFFYGE GSNTYVCKVQ EQIDQERRKV AKACGLHLNS
     LLQECNDEYG TDYATLREYC LAPSPHNVHY ACPDNMEHRY FSEELCSLED IAAIATITKI
     EMPLTRAFIN IIHAGKGNFP PTDKTSSVIG NFRSGDLIRF GATIFVKDEK MK