ID   DHMH_METME              Reviewed;         405 AA.
AC   Q59542;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Methylamine dehydrogenase heavy chain;
DE            Short=MADH;
DE            EC=1.4.9.1;
DE   AltName: Full=Methylamine dehydrogenase (amicyanin);
DE   Flags: Precursor;
GN   Name=mauB;
OS   Methylophilus methylotrophus (Bacterium W3A1).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylophilus.
OX   NCBI_TaxID=17;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8021188; DOI=10.1128/jb.176.13.4073-4080.1994;
RA   Chistoserdov A.Y., McIntire W.S., Mathews F.S., Lidstrom M.E.;
RT   "Organization of the methylamine utilization (mau) genes in Methylophilus
RT   methylotrophus W3A1-NS.";
RL   J. Bacteriol. 176:4073-4080(1994).
CC   -!- FUNCTION: Methylamine dehydrogenase carries out the oxidation of
CC       methylamine. Electrons are passed from methylamine dehydrogenase to
CC       amicyanin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + methylamine + 2 oxidized [amicyanin] = formaldehyde + 2
CC         H(+) + NH4(+) + 2 reduced [amicyanin]; Xref=Rhea:RHEA:30207,
CC         Rhea:RHEA-COMP:11100, Rhea:RHEA-COMP:11101, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16842, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29036, ChEBI:CHEBI:49552, ChEBI:CHEBI:59338; EC=1.4.9.1;
CC   -!- SUBUNIT: Tetramer of two light and two heavy chains.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- SIMILARITY: Belongs to the aromatic amine dehydrogenase heavy chain
CC       family. {ECO:0000305}.
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DR   EMBL; L26407; AAB46948.1; -; Genomic_DNA.
DR   PIR; T10070; T10070.
DR   AlphaFoldDB; Q59542; -.
DR   SMR; Q59542; -.
DR   STRING; 1122236.KB905144_gene2358; -.
DR   PRIDE; Q59542; -.
DR   BioCyc; MetaCyc:MON-3907; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0030058; F:amine dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0052876; F:methylamine dehydrogenase (amicyanin) activity; IEA:UniProtKB-EC.
DR   GO; GO:0030416; P:methylamine metabolic process; IEA:InterPro.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR013476; MeN_DH_Hvc.
DR   InterPro; IPR009451; Metamine_DH_Hvc.
DR   InterPro; IPR011044; Quino_amine_DH_bsu.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   Pfam; PF06433; Me-amine-dh_H; 1.
DR   SUPFAM; SSF50969; SSF50969; 1.
DR   TIGRFAMs; TIGR02658; TTQ_MADH_Hv; 1.
PE   3: Inferred from homology;
KW   Electron transport; Oxidoreductase; Periplasm; Signal; Transport.
FT   SIGNAL          1..36
FT                   /evidence="ECO:0000255"
FT   CHAIN           37..405
FT                   /note="Methylamine dehydrogenase heavy chain"
FT                   /id="PRO_0000025579"
SQ   SEQUENCE   405 AA;  45244 MW;  D7B0FB8842749E94 CRC64;
     MTTFDHPSMI RQPKPTGLAG GLVLAALMLS SSLALADATP NQLGSEITAK LQDETSIAIA
     PASDSKRVYV LDPGNFHMTS TVYTIDGKSS KLLGMTDAGK LPNVMVASDG KFLAIANTMY
     SRVARGKRDD YLELIDTKTH QPIADIDIPE GRFLTGVFER TAGLSVDDKH LLFQQFSPSP
     GVGLVDLQQK AFVKIMNVPD CYHIFPTANQ NFFMHCRDGS LMQFTYDSKG NTKQKPTKIF
     HAEKEYLLNN PYYSNSNNHL TWPTYEGKIF QAKLSESGAE FLKPIEVFTD KEKADKWRPG
     GWQTIAFHKA RNELYLLADQ REKWTHKLPS RFVFVVDATS GKRLRRIELK HEINSIAVSQ
     DDKPYLYAVS EEAKTLFTFD AVNGKALSSI DELGRAPSMI FIADK