ID   DHMA_CAUVN              Reviewed;         302 AA.
AC   B8H3S9;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01230};
DE            EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01230};
GN   Name=dhmA {ECO:0000255|HAMAP-Rule:MF_01230}; OrderedLocusNames=CCNA_01233;
OS   Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=565050;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA1000 / CB15N;
RX   PubMed=20472802; DOI=10.1128/jb.00255-10;
RA   Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA   Walunas T.L., Crosson S.;
RT   "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL   J. Bacteriol. 192:3678-3688(2010).
CC   -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC       halogenated aliphatic compounds, leading to the formation of the
CC       corresponding primary alcohols, halide ions and protons.
CC       {ECO:0000255|HAMAP-Rule:MF_01230}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC         H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01230};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01230}.
CC   -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 1
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01230}.
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DR   EMBL; CP001340; ACL94698.1; -; Genomic_DNA.
DR   RefSeq; WP_010919059.1; NC_011916.1.
DR   RefSeq; YP_002516606.1; NC_011916.1.
DR   AlphaFoldDB; B8H3S9; -.
DR   SMR; B8H3S9; -.
DR   ESTHER; caucr-CC1175; Haloalkane_dehalogenase-HLD1.
DR   PRIDE; B8H3S9; -.
DR   EnsemblBacteria; ACL94698; ACL94698; CCNA_01233.
DR   GeneID; 7333626; -.
DR   KEGG; ccs:CCNA_01233; -.
DR   PATRIC; fig|565050.3.peg.1215; -.
DR   HOGENOM; CLU_020336_13_3_5; -.
DR   OMA; TLFCQDW; -.
DR   OrthoDB; 1890883at2; -.
DR   PhylomeDB; B8H3S9; -.
DR   Proteomes; UP000001364; Chromosome.
DR   GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   HAMAP; MF_01230; Haloalk_dehal_type1; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   InterPro; IPR023489; Haloalkane_dehalogenase_1.
DR   Pfam; PF12697; Abhydrolase_6; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..302
FT                   /note="Haloalkane dehalogenase"
FT                   /id="PRO_1000164971"
FT   DOMAIN          48..152
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        123
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT   ACT_SITE        249
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT   ACT_SITE        278
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
SQ   SEQUENCE   302 AA;  33155 MW;  B0B8C27EE8D9A97C CRC64;
     MDVLRTPDER FEGLADWSFA PHYTEVTDAD GTALRIHHVD EGPKDQRPIL LMHGEPSWAY
     LYRKVIAELV AKGHRVVAPD LVGFGRSDKP AKRTDYTYER HVAWMSAWLE QNDLKDIVLF
     CQDWGGLIGL RLVAAFPERF SAVVVSNTGL PIGVGKSEGF EAWLNFSQNT PELPVGFILN
     GGTARDLSDA ERSAYDAPFP DESYKEGARI FPALVPITPE HASVEENKAA WAVLETFDKP
     FVTAFSDADP ITRGGEAMFL ARVPGTKNVA HTTLKGGHFV QEDSPVEIAA LLDGLVAGLP
     QA