DFD_DROME
ID DFD_DROME Reviewed; 586 AA.
AC P07548; Q9UAL9; Q9VI48;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 198.
DE RecName: Full=Homeotic protein deformed;
GN Name=Dfd; ORFNames=CG2189;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=16453752; DOI=10.1002/j.1460-2075.1987.tb04819.x;
RA Regulski M., McGinnis N., Chadwick R., McGinnis W.;
RT "Developmental and molecular analysis of Deformed: a homeotic gene
RT controlling Drosophila head development.";
RL EMBO J. 6:767-777(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Berkeley;
RA Celniker S.E., Pfeiffer B.D., Knafels J., Martin C.H., Mayeda C.A.,
RA Palazzolo M.J.;
RT "Complete sequence of the Antennapedia complex of Drosophila.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 163-586.
RX PubMed=3938363; DOI=10.1101/sqb.1985.050.01.032;
RA Laughon A., Carroll S.B., Storfer F.A., Riley P.D., Scott M.P.;
RT "Common properties of proteins encoded by the Antennapedia complex genes of
RT Drosophila melanogaster.";
RL Cold Spring Harb. Symp. Quant. Biol. 50:253-262(1985).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 366-482.
RX PubMed=2416463; DOI=10.1016/0092-8674(85)90013-3;
RA Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W.;
RT "Homeo box genes of the Antennapedia and bithorax complexes of
RT Drosophila.";
RL Cell 43:71-80(1985).
RN [7]
RP FUNCTION.
RX PubMed=12202035; DOI=10.1016/s0092-8674(02)00871-1;
RA Lohmann I., McGinnis N., Bodmer M., McGinnis W.;
RT "The Drosophila Hox gene deformed sculpts head morphology via direct
RT regulation of the apoptosis activator reaper.";
RL Cell 110:457-466(2002).
RN [8]
RP REVIEW ON FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12492146; DOI=10.1046/j.1525-142x.2002.02034.x;
RA Hughes C.L., Kaufman T.C.;
RT "Hox genes and the evolution of the arthropod body plan.";
RL Evol. Dev. 4:459-499(2002).
CC -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC developmental regulatory system that provides cells with specific
CC positional identities on the anterior-posterior axis. Homeotic protein
CC controlling Drosophila head development. Transcriptional activator of
CC the apoptotic activator protein rpr in cells at the
CC maxillary/mandibular boundary. {ECO:0000269|PubMed:12202035,
CC ECO:0000269|PubMed:16453752}.
CC -!- INTERACTION:
CC P07548; P40427: exd; NbExp=2; IntAct=EBI-458925, EBI-101537;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the mandibular and maxillary segments
CC of the head, with some expression in the posterior intercalary segment
CC which become part of the CNS. In the imaginal tissues, expressed in
CC part of the eye antennal disk which give rise to portions of the head
CC capsule and to a small patch of expression in the basal-most portion of
CC the labial disk. {ECO:0000269|PubMed:12492146}.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily.
CC {ECO:0000305}.
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DR EMBL; X05136; CAA28782.1; -; Genomic_DNA.
DR EMBL; AE001572; AAD19796.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54083.2; -; Genomic_DNA.
DR EMBL; M13373; AAA28375.1; -; mRNA.
DR PIR; A26638; A26638.
DR RefSeq; NP_477201.1; NM_057853.3.
DR AlphaFoldDB; P07548; -.
DR SMR; P07548; -.
DR BioGRID; 66030; 41.
DR DIP; DIP-18055N; -.
DR IntAct; P07548; 20.
DR STRING; 7227.FBpp0081138; -.
DR PaxDb; P07548; -.
DR PRIDE; P07548; -.
DR DNASU; 40832; -.
DR EnsemblMetazoa; FBtr0081621; FBpp0081138; FBgn0000439.
DR GeneID; 40832; -.
DR KEGG; dme:Dmel_CG2189; -.
DR UCSC; CG2189-RA; d. melanogaster.
DR CTD; 40832; -.
DR FlyBase; FBgn0000439; Dfd.
DR VEuPathDB; VectorBase:FBgn0000439; -.
DR eggNOG; KOG0489; Eukaryota.
DR HOGENOM; CLU_468753_0_0_1; -.
DR InParanoid; P07548; -.
DR OMA; GMTGHPH; -.
DR OrthoDB; 858478at2759; -.
DR PhylomeDB; P07548; -.
DR SignaLink; P07548; -.
DR BioGRID-ORCS; 40832; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 40832; -.
DR PRO; PR:P07548; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000439; Expressed in mouthpart and 27 other tissues.
DR ExpressionAtlas; P07548; baseline and differential.
DR Genevisible; P07548; DM.
DR GO; GO:0043025; C:neuronal cell body; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:1990403; P:embryonic brain development; IMP:FlyBase.
DR GO; GO:0060322; P:head development; IMP:FlyBase.
DR GO; GO:0060323; P:head morphogenesis; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:1902339; P:positive regulation of apoptotic process involved in morphogenesis; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0035289; P:posterior head segmentation; IMP:FlyBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007380; P:specification of segmental identity, head; IMP:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017995; Homeobox_antennapedia.
DR InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00025; ANTENNAPEDIA.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00032; ANTENNAPEDIA; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..586
FT /note="Homeotic protein deformed"
FT /id="PRO_0000200261"
FT DNA_BIND 366..425
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 90..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 492..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 343..348
FT /note="Antp-type hexapeptide"
FT COMPBIAS 109..137
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..332
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..479
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..565
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 32
FT /note="L -> V (in Ref. 1; CAA28782)"
FT /evidence="ECO:0000305"
FT CONFLICT 227
FT /note="S -> I (in Ref. 5; AAA28375)"
FT /evidence="ECO:0000305"
FT CONFLICT 442
FT /note="G -> A (in Ref. 6)"
FT /evidence="ECO:0000305"
FT CONFLICT 443
FT /note="N -> K (in Ref. 5; AAA28375)"
FT /evidence="ECO:0000305"
FT CONFLICT 456
FT /note="Missing (in Ref. 6)"
FT /evidence="ECO:0000305"
FT CONFLICT 477
FT /note="T -> TQQT (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 541
FT /note="N -> NN (in Ref. 1; CAA28782)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 586 AA; 63380 MW; 9A7C79C744FAFF18 CRC64;
MSSFLMGYPH APHHVQSPMS MGNGLDPKFP PLADDYHHYN GHYSMTASTG HMSGAVGGGA
GVGSVGGGGA GGMTGHPHSM HPADMVSDYM AHHHNPHSHS HSHTHSLPHH HSNSAISGHH
QASAGGYSSN YANATPPSHP HSHPHAHPHQ SLGYYVHHAP EFISAGAVHS DPTNGYGPAA
NVPNTSNGGG GGGSGAVLGG GAVGGSANGY YGGYGGGYGT ANGSVGSTHS QGHSPHSQMM
DLPLQCSSTE PPTNTALGLQ ELGLKLEKRI EEAVPAGQQL QELGMRLRCD DMGSENDDMS
EEDRLMLDRS PDELGSNDND DDLGDSDSDE DLMAETTDGE RIIYPWMKKI HVAGVANGSY
QPGMEPKRQR TAYTRHQILE LEKEFHYNRY LTRRRRIEIA HTLVLSERQI KIWFQNRRMK
WKKDNKLPNT KNVRKKTVDA NGNPTPVAKK PTKRAASKKQ QQAQQQQQSQ QQQTQQTPVM
NECIRSDSLE SIGDVSSSLG NPPYIPAAPE TTSSYPGSQQ HLSNNNNNGS GNNNNNNNNN
NSNLNNNNNN NQMGHTNLHG HLQQQQSDLM TNLQLHIKQD YDLTAL
