PNPP(对硝基苯基磷酸酯)二钠是用于 ELISA 和常规分光光度测定的碱性和酸性磷酸酶的非蛋白质显色底物。
Cas No. | 4264-83-9 |
别名 | 4-硝基苯磷酸二钠,Para-nitrophenyl phosphate disodium |
Canonical SMILES | O=P([O-])([O-])OC1=CC=C([N+]([O-])=O)C=C1.[Na+].[Na+] |
分子式 | C6H4NNa2O6P |
分子量 | 263.05 |
溶解度 | Water : 160 mg/mL (608.25 mM) |
储存条件 | Store at -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays. Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm[1]. |